摘要
背景和目的：屠宰场及其废水可能成为环境中抗生素耐药性细菌发生和分布的“热点”。本研究旨在了解从印度尼西亚万丹省生猪屠宰场地板表面和污水样本中分离的大肠杆菌中四环素抗性基因的分布。
材料和方法：从万丹省的10家生猪屠宰场采集10个样本，每个样本来自地板表面拭子和废水。参考世界卫生组织（2021年）的全球三环监测扩展谱β-内酰胺酶大肠杆菌协议，分离并鉴定了大肠杆菌菌株。采用实时定量聚合酶链反应（qPCR）检测tet基因。
结果：tetA、tetB、tetC、tetM、tet0和tetX基因分布在地板表面样品的分离物中，tetA，tetC，tetE，tetM，teT0和tetX基因分布在出水样品的分离株中。tetO基因（60%）在来自地面样品的分离物中是最显性的基因，而tetA基因在来自出水样品的分离株中是显性的基因（50%）。tetA+tetO基因组合是大肠杆菌分离株中的显性模式（15%）。
结论：tet基因在屠宰场地面和污水样本中的高流行率和多样性
在万丹省的研究表明，tet基因已经从猪传播到环境中；因此，这个
这种情况应该被视为对公众健康的严重威胁。
Abstract
Background and Aim: Slaughterhouses and their effluents could serve as a “hotspot” for the occurrence and distribution
of antibiotic-resistant bacteria in the environment. This study aimed to understand the distribution of tetracycline resistance
genes in Escherichia coli isolated from the floor surface and effluent samples of pig slaughterhouses in Banten Province,
Indonesia.
Materials and Methods: Ten samples, each from floor surface swabs and effluents, were collected from 10 pig
slaughterhouses in Banten Province. Escherichia coli strains were isolated and identified by referring to the protocol of
the Global Tricycle Surveillance extended-spectrum beta-lactamase E. coli from the WHO (2021). Quantitative real-time
polymerase chain reaction (qPCR) was used to detect the tet genes.
Results: The tetA, tetB, tetC, tetM, tetO, and tetX genes were distributed in the isolates from the floor surface samples,
and the tetA, tetC, tetE, tetM, tetO, and tetX genes were distributed in the isolates from the effluent samples. The tetO gene
(60%) was the most dominant gene in the isolates from floor surface samples, while the tetA gene was the dominant one
in the isolates from the effluent samples (50%). The tetA + tetO gene combination was the dominant pattern (15%) in the
E. coli isolates.
Conclusion: The high prevalence and diversity of the tet genesin floor surface and effluent samples from pig slaughterhouses
in Banten Province indicated that the transmission of the tet genes had occurred from pigs to the environment; thus, this
situation should be considered a serious threat to public health.

http://www.veterinaryworld.org/Vol.16/March-2023/11.pdf