摘要
      耐多药溶血葡萄球菌是医院常见的侵袭性菌血症病原体。我们之前的分析表明，主要菌株之一，源自ST3的ST42，在SH1431的7个基因座中只有一个多点序列分型（MLST）变异；然而在ST42和ST3之间没有观察到生物膜形成的显著差异，这表明其他因素影响克隆谱系的变化。对ST42和ST3的两个分离株进行全基因组测序，以发现表型和基因型变异，并在140个临床分离株中进一步验证了这些变异。梭形酸和四环素抗性基因（fusB和tetK）仅在CGMH-SH51（ST42）中发现。进一步的研究显示，所有分离株的抗性基因型一致，分别有46%和70%的ST42含有fusB和tetK。相反，只有23%和4.2%的ST3分别含有这两个基因。表型分析还表明，与ST3（分别为23%和4%）相比，ST42分离株对fusidic酸（47%）和四环素（70%）具有高度抗性。除了耐药基因外，ST42中的三个胶囊相关基因的百分比分布高于ST3分离株。我们的研究结果表明，ST42可能在台湾流行，需要进一步的组成监测来防止这种细菌的传播。
Abstract
Multi-drug resistant Staphylococcus haemolyticus is a frequent nosocomial invasive bacteremia pathogen in hospitals. Our previous analysis showed one of the predominant strains, ST42 originated from ST3, had only one multilocus sequence typing (MLST) variation among seven loci in SH1431; yet no significant differences in biofilm formation observed between ST42 and ST3, suggesting that other factors influence clonal lineage change. Whole genome sequencing was conducted on two isolates from ST42 and ST3 to find phenotypic and genotypic variations, and these variations were further validated in 140 clinical isolates. The fusidic acid- and tetracycline-resistant genes (fusB and tetK) were found only in CGMH-SH51 (ST42). Further investigation revealed consistent resistant genotypes in all isolates, with 46% and 70% of ST42 containing fusB and tetK, respectively. In contrast, only 23% and 4.2% ST3 contained these two genes, respectively. The phenotypic analysis also showed that ST42 isolates were highly resistant to fusidic acid (47%) and tetracycline (70%), compared with ST3 (23% and 4%, respectively). Along with drug-resistant genes, three capsule-related genes were found in higher percentage distributions in ST42 than in ST3 isolates. Our findings indicate that ST42 could become endemic in Taiwan, further constitutive surveillance is required to prevent the spread of this bacterium.

https://www.mdpi.com/1422-0067/24/7/6198