摘要
      抗生素耐药菌的繁殖严重危害生态安全和人类健康。本研究采用黄铁矿（FeS2，天然丰富的矿物）进行高碘酸盐（PI）活化以消毒ARB。FeS2/PI系统可以在20分钟内对1×107CFU mL−1的卡那霉素抗性大肠杆菌进行消毒，使其低于检测极限。在pH为3至9、离子强度为0至300mM、悬浮液中HA（0.1–10 mg L−1）以及自来水、河水和污水等真实水样中，都能实现有效的ARB灭活性能。FeS2/PI系统也能有效地对庆大霉素耐药大肠杆菌和革兰氏阳性枯草芽孢杆菌进行消毒。产生的反应物种包括Fe（IV）、·O2–和·OH，会攻击细胞膜并淹没细胞内防御系统。细胞内的卡那霉素抗性基因在FeS2/PI系统中被释放并降解。PI优选吸附在FeS2的Fe位点上（具有较低的吸附能、较多的键合态占有率和较强的键合强度）。随后电子云从Fe位点转移到PI将裂解Isingle-bond-O键以产生反应物种。此外，FeS2/PI系统还可以与砂滤系统相结合，有效地捕获和消毒ARB。因此，FeS2/PI系统是一种在不同情况下灭活ARB的有前途的方法。
Abstract
The propagation of antibiotic-resistant bacteria (ARB) greatly endangers the ecological safety and human health. This study employed pyrite (FeS2, naturally abundant mineral) for periodate (PI) activation to disinfect ARB. FeS2/PI system could disinfect 1 × 107 CFU mL−1 of kanamycin-resistant E.coli below the limit of detection in 20 min. Efficient ARB inactivation performance was achieved in pH from 3 to 9, ionic strength from 0 to 300 mM, with HA (0.1–10 mg L−1) in suspension, and in real water samples including tap water, river water and sewage. FeS2/PI system could also efficiently disinfect gentamycin-resistant E.coli and Gram-positive B. subtilis. The generated reactive species including Fe(IV), ·O2– and ·OH would attack cell membrane and overwhelmed intracellular defense system. The intracellular kanamycin resistance genes in cells would be released and then degraded in FeS2/PI system. PI preferred to be adsorbed on Fe site of FeS2 (with lower adsorption energy, more occupancy of bonding state and stronger bonding strength). The subsequent transfer of electron cloud from Fe site to PI would cleave Isingle bondO bond to generate reactive species. Moreover, FeS2/PI system could also combine with sand filtration system to efficiently capture and disinfect ARB. Therefore, FeS2/PI system is a promising approach to inactivate ARB in different scenarios.

https://www.sciencedirect.com/science/article/abs/pii/S0043135422014531