摘要：
      按照规定的程序采集了三个水体的水生生物样本。使用曙红亚甲基蓝琼脂（EMB）、甘露醇盐琼脂（MSA）、柠檬酸硫代硫酸盐胆汁盐蔗糖琼脂（TCBS）、Muller Hinton琼脂（MH）分离细菌。对分离株进行了菌落、形态和生化鉴定。用血琼脂对分离株进行致病性试验，测定其溶血活性。采用Kirby-Bauer纸片扩散法对标准抗生素的耐药性状况进行了调查，并测定了分离株的多种抗生素耐药性指标。确定质粒图谱，并使用分子手段鉴定抗性基因。分离到的细菌种类为韦氏菌、东方弧菌和黄微球菌。Veillonella Spp显示β溶血，而Microccus luteus显示α溶血，Vibrio orientalis显示γ溶血。抗生素敏感性测试显示，所有分离株都有不同程度的抗生素耐药性，并且根据其耐药性水平，有多种抗生素耐药性指数（MARi）。韦略内拉。spp.具有0.3的MARi，而M.luteus和V.orietalis具有0.5的MARi。质粒图谱显示所有分离株都具有带宽为10000bp的质粒。β-内酰胺酶基因（bla SHV）阳性。只有弧菌对氨基糖苷类（aac（3）-IV）的耐药性呈阳性。东方弧菌（Vibrio orientalis）和黄微球菌（Microccus luteus）具有对喹诺酮类药物的耐药性基因（qnrA基因），而所有分离株都具有对所用磺酰胺类药物的抗性基因（sul1基因）。这项工作的结果确定了从水生栖息地分离的细菌的抗生素耐药性状况，并表明它们在抗生素耐药性AMR和抗生素耐药性基因（ARGs）传播中的重要作用
Abstract: 
Aquatic samples from three water bodies were collected following laid down procedures. The bacteria were isolated using, Eosin Methylene Blue agar (EMB), Mannitol salt agar (MSA), Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar, Muller Hinton (MH). The isolates were subjected to colonial, morphological and biochemical characterization. Pathogenicity test was carried out on the isolates using blood agar to determine their hemolytic activities. Antibiotic resistance status was also investigated against standard antibiotics using the Kirby Bauer disk diffusion method and the multiple antibiotic resistance indices of the isolates were also determined. The plasmid profiles were determined and the resistance genes were identified using molecular means. The bacteria species isolated were Veillonella Spp, Vibrio orientalis and Micrococcus luteus. Veillonella Spp showed Beta hemolysis while Micrococcus luteus showed alpha hemolysis and Vibrio orientalis showed gamma hemolysis. Antibiotic susceptibility test showed all isolates to be antibiotic resistant to varying degrees and multiple antibiotic resistance indices (MARi) depending on their level of resistance. Veillonella. spp. had MARi of 0.3 while M. luteus and V. orietalis had MARi of 0.5. Plasmid profiling showed all isolates to have plasmids with bandwidth of 10,000 bp. Vibrio spp and Veillonella Spp were positive for beta lactamase genes (bla SHV). Only Vibrio was positive for resistance to aminoglycosides (aac(3)-IV). Vibrio orientalis and Micrococcus luteus were found to have the resistance genes for quinolones (qnrA gene) while all the isolates possess genes against the sulphonamides (sul1 gene) used. The result of this work establishes the antibiotic resistance status of bacteria isolated from aquatic habitat and suggests their important role in the spread of antimicrobial resistance AMR, and antibiotic resistance genes (ARGs)

https://gsconlinepress.com/journals/gscbps/content/resistance-genes-and-plasmid-profile-bacteria-isolated-selected-water-bodies-owo-metropolis