摘要
      在本研究中，研究了混凝/絮凝和芬顿工艺的组合作为三级处理，以产生易于重复使用的处理水。这两种工艺的结合从未应用于真正的城市污水消毒。当使用120 mg/L的混凝剂（FeCl3）和出水的自然pH（7.14）时，浊度和肠道细菌的去除效果最好。当使用过氧化氢和添加的铁浓度分别为100 mg/L和7 mg/L时，以下芬顿反应在25°C下120分钟后表现出最大的肠道细菌灭活。在每个治疗步骤前后，评估抗生素耐药性（阿莫西林和磺胺甲恶唑）肠道细菌和总肠道细菌、肠球菌和异养菌以及抗生素耐药性基因ARG-（sul1、blaTEM和qnrS）的丰度。在处理后和储存72小时后，总的和耐药的可培养肠道细菌的值立即低于10 CFU/100 mL，因此达到了对大肠杆菌的最严格限制。物理化学参数也符合水再利用的既定限制。尽管含有丰富多样的细菌群落，但最终储存的消毒废水中含有相对丰度较高的潜在危险细菌。这些结果指出，需要对处理过的废水进行深入的微生物表征，以评估其在灌溉中重复使用的风险。
Abstract
In this study, a combination of coagulation/flocculation and Fenton processes was studied as tertiary treatment in order to generate treated water susceptible to reuse. The combination of both processes has never been applied in disinfection of real urban wastewater. The best removals of turbidity and enterobacteria were achieved when applying a coagulant (FeCl3) dosage of 120 mg/L and the natural pH of the effluent (7.14). The following Fenton reaction presented the maximal enterobacteria inactivation after 120 min at 25 °C, when using hydrogen peroxide and added iron concentrations of 100 mg/L and 7 mg/L, respectively. The abundance of antibiotic resistant (amoxicillin and sulfamethoxazole) enterobacteria and total enterobacteria, enterococci, and heterotrophs, and antibiotic resistance genes - ARG - (sul1, blaTEM and qnrS) was evaluated before and after each step of the treatment. Values below 10 CFU/100 mL were achieved for total and resistant cultivable enterobacteria immediately after treatment and after storage for 72 h, therefore meeting the strictest limit imposed for E. coli. Physico-chemical parameters also met the established limits for water reuse.

Despite harbouring a rich and diverse bacterial community, the final stored disinfected wastewater contained high relative abundance of potentially hazardous bacteria. Such results point out the need of a deep microbiological characterization of treated wastewater to evaluate the risk of its reuse in irrigation.

https://www.sciencedirect.com/science/article/abs/pii/S0013935122023556