摘要
      该研究通过qPCR定量了德国23个不同污水处理厂废水中抗生素抗性基因（ARGs）和兼性致病菌（FPB）的丰度，以及基因组DNA中的一个移动遗传元件。将12种临床相关的ARGs分为常见、中期和罕见的公共废水遗传参数。对5种针对大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌、鲍曼不动杆菌和肠球菌的FPB进行了分类PCR定量。污水处理厂的集水区不同，仅受医院、食品加工公司或住房区的影响。研究发现，分析的ARG和FPB的总排放量与污水处理厂的大小无关，一个集群内的最大差异为两个对数单位。最初，定量数据评估显示ARG类别和污水处理厂集水区之间没有显著差异。Pearson相关性方法使更明显的相关性变得明显，其中将每个单独的分类标记与每个ARG靶标进行比较。在受医院废水影响的污水处理厂废水中，发现FPB（即大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和肠球菌）与罕见耐药基因（blaNDM-1、vanA）类临床相关ARG的相关性增加。
Abstract
The study quantified the abundances of antibiotic resistance genes (ARGs) and facultative pathogenic bacteria (FPB) as well as one mobile genetic element in genomic DNA via qPCR from 23 different wastewater treatment plant (WWTP) effluents in Germany. 12 clinically relevant ARGs were categorized into frequently, intermediately, and rarely occurring genetic parameters of communal wastewaters. Taxonomic PCR quantifications of five FPB targeting Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, and enterococci were performed. The WWTPs differed in their catchment areas being impacted by hospitals, food processing companies, or housing areas only. The total discharges of the analyzed ARGs and FPB were found to cluster independently of the sizes of the WWTPs with a maximum difference of two log units within one cluster. Initially, quantitative data evaluations revealed no significant difference between ARG categories and WWTP catchment areas. More distinct correlations became obvious with a Pearson correlation approach, where each single taxonomic marker is compared to each ARG target. Here, increased correlation of FPB (i.e. E. coli, K. pneumoniae, P. aeruginosa, and enterococci) with clinically relevant ARGs of the category of rarely occurring resistance genes (blaNDM-1, vanA) was found in WWTP effluents being influenced by hospital wastewaters.

https://www.ifg.kit.edu/7602_7922.php