摘要

外部环境在形成肠道微生物组的结构中起关键作用。一种潜在的健康威胁在于从交叉污染的微生物群中释放抗生素抗性基因（ARG）。我们将这项研究集中在农场工人，当地村民和猪的粪便微生物组成和抗生素抗性基因的比较上。我们对这些研究使用了16S rRNA和实时PCR的高通量下一代测序。我们的结果表明，与当地村民相比，工人的物种多样性较低。此外，农场工人的细菌群落，当地村民和猪粪便被明确划分为三组。与猪和当地村民相比，工人们有更多的变形菌。工人和猪的梭菌科比当地村民更为丰富。此外，农场工人或当地村民与猪粪之间存在ARG差异。农业工人和当地村民除了大环内酯ARGs外，其他类似的相对丰度。总之，这些数据表明猪场环境影响猪场工人的粪便细菌组成。然而，ARG传播受猪场环境独立因素的影响。

The external environment plays a critical role in shaping the structure of the gut microbiome. One potential health threat lies in the release of antibiotic resistant genes (ARGs) from cross-contaminated microbiomes. We focused this study on a comparison of fecal microbial composition and antibiotic resistant genes between farm workers, local villagers and swine. We used a high-throughput next-generation sequencing of 16S rRNA and real-time PCR for these studies. Our results indicated that workers had less species diversity as compared to the local villagers. Moreover, the bacterial communities of the farm workers, the local villagers and swine feces were clearly divided into three groups. The workers had a greater abundance of Proteobacteria as compared to swine and the local villagers. The Clostridiaceae in the workers and swine were more abundant than the local villagers. In addition, there were ARG differences between the farm workers or local villager’s and swine feces. The farm workers and the local villagers had similar relative abundance except for macrolide ARGs. Taken together, these data suggest that the swine farm environment affects the fecal bacterial composition of swine farm workers. However, ARG spread was influenced by factors independent of the swine farm environment.

https://www.nature.com/articles/s41598-017-04672-y