摘要
在这项研究中，在两个城市污水处理厂（污水处理厂）中对抗生素抗性基因（ARG）的定量和定性变化进行了调查，以处理预处理的牲畜或工业废水以及市政污水。对8个ARG（tetX，tetM，tetA，sul1，sul2，ermB，qnrD和blaTEM）进行定量，并且通过ARGs拷贝/ 16S rRNA基因拷贝评估它们的相对丰度。据观察，两个污水处理厂的ARGs的命运不同：在处理过程中，只有在接受过预处理的畜禽废水的WWTP1中，sul，qnrD和blaTEM增殖。此外，在生物学，二次沉淀和凝血过程中观察到ARGs发生模式的动态变化。在两个污水处理厂的生物处理过程中，tet和ermB的相对丰度发生了变化：tet增加了211.6-357.6％，而ermB降低了70.4-92.0％。在sul，qnrD和blaTEM中观察到很小的变化。随后，在两个污水处理厂的二次沉淀和混凝期间，tet的相对丰度降低：沉淀期间tet分别降低56.0-86.3％和凝固期间降低48.2-75.7％。在最终治疗期间，发现抗生素抗性细菌（ARB）和ARG对紫外线（UV）消毒的不同反应：在34-75％的范围内观察到ARB的去除效率，而在紫外线剂量为27 mJ / cm2。虽然ARG经历了各种处理过程，但是从污水处理厂1排放的相当数量的ARG仍然分别为4.2×1018个拷贝/天和从WWTP2每天5.4×1016个拷贝。
In this study, quantitative and qualitative changes in antibiotics resistance genes (ARGs) were investigated in two municipal wastewater treatment plants (WWTPs) treating pretreated livestock or industrial wastewater as well as municipal sewage. Total eight ARGs (tetX, tetM, tetA, sul1, sul2, ermB, qnrD, and blaTEM) were quantified, and their relative abundance was assessed by ARGs copies/16S rRNA gene copies. The fate of ARGs was observed to be different between two WWTPs: sul, qnrD, and blaTEM were proliferated during the treatment processes only in the WWTP1 which received pretreated livestock wastewater. Furthermore, dynamic shifts in patterns of ARGs occurrence were observed during biological, secondary sedimentation and coagulation processes. During biological treatment in both WWTPs, relative abundance of tet and ermB changed: tetincreased significantly by 211.6–357.6%, while ermB decreased by 70.4–92.0%. Little variation was observed in sul, qnrD and blaTEM. Subsequently, the relative abundance oftet decreased during the secondary sedimentation and coagulation in both WWTPs: tetdecreased by 56.0–86.3% during sedimentation and by 48.2–75.7% during coagulation, respectively. During the final treatment, different responses of antibiotic resistance bacteria (ARB) and ARGs to ultraviolet (UV) disinfection were found: removal efficiencies of ARB were observed in the range of 34–75%, while obvious reduction in ARGs was not observed at the UV dose of 27 mJ/cm2. Although ARGs underwent various treatment processes, considerable levels of ARGs remained at discharge amounting to 4.2 × 1018copies/day from WWTP1 and 5.4 × 1016 copies/day from WWTP2, respectively.
https://www.sciencedirect.com/science/article/pii/S0048969717316704
