摘要

由于抗生素和重金属过度用于畜牧业，动物粪便是抗生素抗性基因（ARG）的库。据报道，厌氧消化有可能减少ARGs。然而，很少有研究调查厌氧消化过程中ARGs的减少是否会受到包括抗生素和重金属在内的不同外部压力的影响。因此，本研究的目的是研究金霉素（CTC）和Cu在猪粪厌氧消化过程中对ARG，重金属抗性基因（HMRG）和活动遗传元件（MGE）的还原作用。结果表明，通过嗜温厌氧消化可以有效地减少主要的ARG（tetO，tetW，tetX，tetL）（约1.00log拷贝/ g TS）。微生物群落演化是主要驱动力。有趣的是，密螺旋体可能表明终止厌氧消化并与ARGs宿主细菌竞争。 CTC，Cu和CTC + Cu的添加影响微生物群落的变化，阻碍了ARGs的去除，特别是CTC + Cu在厌氧消化过程中严重影响了Treponema和ARGs。

As antibiotic and heavy metals are over used in the livestock industry, animal manure is a reservoir of antibiotic resistance genes (ARGs). Anaerobic digestion has been reported to have the potential to reduce ARGs. However, few studies investigated whether reduction of ARGs would be affected by different external pressures including antibiotics and heavy metals during anaerobic digestion. The purpose of this study was thus to investigate effects of both chlortetracycline (CTC) and Cu on reduction of ARGs, heavy metal resistance genes (HMRGs) and mobile genetic elements (MGEs) during the swine manure anaerobic digestion. The results showed that the predominant ARGs (tetO, tetW, tetX, tetL) could be effectively reduced (approximately 1.00 log copies/g TS) through mesophilic anaerobic digestion. Microbial community evolution was the main driver. It was interesting thatTreponema might indicate the termination of anaerobic digestion and compete with ARGs host bacteria. Addition of CTC, Cu and CTC + Cu affected microbial community change and hindered removal of ARGs, especially, CTC + Cu seriously affected Treponema and ARGs during anaerobic digestion.

https://www.sciencedirect.com/science/article/pii/S0960852417304157