摘要

弯曲杆菌对食品安全和人兽医学具有重要意义。这项研究强调了加纳兽医公共卫生领域的抵抗情况。使用选择性mCCDA琼脂，通过API CAMPY在表型上确认分离株，并通过IpxA基因的多重PCR对基因型进行基因型鉴定。物种对普通和相关抗生素的敏感性谱由Kirby-Bauer纸片扩散法测定。分别分析的牛，绵羊，山羊和猪粪样品产生13.2％（16/121），18.6％（22/102），18.5％（25/135）和28.7％（29/101）弯曲杆菌属物种，而分别从屠体中回收34.5％（38/110），35.9％（42/117），23.9％（32/134）和36.3％（37/102）。在粪便中鉴定的物种是空肠弯曲菌35.8％（33/92），空肠弯曲菌亚种。 4.3％（4/92），大肠杆菌47.8％（44/92）和C. lari 12.0％（11/92）。在尸体中发现的物种是空肠弯曲杆菌83.9％（125/149），空肠弯曲菌亚种。 doylei 2.0％（3/149），大肠杆菌6.0％（9/149）和C. lari 8.1％（12/149）。对β-内酰胺的耐药性范围为92-97％，对喹诺酮类药物为7-69％，对氨基糖苷类药物为0-44％，对红霉素为97-100％，对四环素为48-94％，对氯霉素为45-88％对亚胺培南观察到耐甲氧苄氨嘧啶/磺胺甲恶唑的耐药率为42％〜86％，为0％。

Campylobacter is of major significance in food safety and human and veterinary medicine. This study highlighted resistance situation in the area of veterinary public health in Ghana. Using selective mCCDA agar, isolates were confirmed phenotypically on API CAMPY and genotypically by multiplex PCR of IpxA gene. The susceptibility profile of species to common and relevant antibiotics was determined by the Kirby-Bauer disk diffusion method. Cattle, sheep, goat, and pig faecal samples analysed, respectively, yielded 13.2% (16/121), 18.6% (22/102), 18.5% (25/135), and 28.7% (29/101) Campylobacter species while 34.5% (38/110), 35.9% (42/117), 23.9% (32/134), and 36.3% (37/102) were, respectively, recovered from the carcasses. Species identified in faeces were C. jejuni 35.8% (33/92), C. jejuni subsp. doylei 4.3% (4/92), C. coli 47.8% (44/92), andC. lari 12.0% (11/92). Species discovered in carcasses were C. jejuni 83.9% (125/149), C. jejuni subsp. doylei2.0% (3/149), C. coli 6.0% (9/149), and C. lari 8.1% (12/149). Resistance ranged from 92 to 97% to the β-lactams, 7 to 69% to the quinolones, 0 to 44% to the aminoglycosides, 97 to 100% to erythromycin, 48 to 94% to tetracycline, 45 to 88% to chloramphenicol, and 42 to 86% to trimethoprim/sulfamethoxazole as 0% resistance was observed against imipenem.

https://www.hindawi.com/journals/bmri/2017/4091856/abs/