摘要

β-内酰胺酶数据库（BLDB）是一个综合的，手工策划的公共资源，提供最新的结构和功能信息，重点关注酶的超家族，对抗生素耐药性产生重大影响。在文献中报道和表征的所有酶均按照它们所属的类别（A，B，C和D），家族和亚家族呈现。还显示了存在于蛋白质数据库中的所有β-内酰胺酶的三维结构。代表性突变体和水解图谱（动力学）的表征完成了图像，并且这四个元素共同构成了更好地理解该酶家族内的结构 - 功能关系的基本基础。可以使用不同的基于蛋白质和核苷酸的BLAST搜索来查询BLDB，这代表了在监测抗生素抗性进展的情况下特别重要的关键特征。 BLDB可在http://bldb.eu上在线获得，无需任何注册，并支持所有现代浏览器。

Beta-Lactamase Database (BLDB) is a comprehensive, manually curated public resource providing up-to-date structural and functional information focused on this superfamily of enzymes with a great impact on antibiotic resistance. All the enzymes reported and characterised in the literature are presented according to the class (A, B, C and D), family and subfamily to which they belong. All three-dimensional structures of β-lactamases present in the Protein Data Bank are also shown. The characterisation of representative mutants and hydrolytic profiles (kinetics) completes the picture and altogether these four elements constitute the essential foundation for a better understanding of the structure-function relationship within this enzymes family. BLDB can be queried using different protein- and nucleotide-based BLAST searches, which represents a key feature of particular importance in the context of the surveillance of the evolution of the antibiotic resistance. BLDB is available online at http://bldb.eu without any registration and supports all modern browsers.

https://www.tandfonline.com/doi/abs/10.1080/14756366.2017.1344235