摘要

沙门氏菌是食源性疾病的主要原因之一。食物，特别是动物来源的食物，是沙门氏菌传播的重要角色。在这项研究中，对从8个省的零售生鸡胴体中回收的743株沙门氏菌分离株的抗生素敏感性进行了测试，并且还筛选了分离株中是否存在I类整合子和耐药基因盒。一百三十（15.21％）的分离物窝藏I类整合子。四川省零售鸡肉中整合子阳性沙门氏菌株的比例较高（29.33％），其次是北京（22.14％），陕西（19.15％），广西（14.13％），河南（12.50％），上海7.25％），福建（8.22％）和广东（6.25％）。在大型，免费和小型市场从零售鸡中回收的沙门氏菌分离株中I类整合子的相应流行率分别为16.31％，14.04％和15.27％。此外，分别从冷藏，冷藏和环境条件下储存的零售鸡中回收的沙门氏菌分离株的20.13％，14.02％和13.74％均为I类整合子阳性。随后对I类整合子进行测序，发现存在10个带有抗性基因的基因盒（dfrA17-aadA5，dfrA17-aadA5，dfrA1-aadA1，dfrA12-aadA2，dfrA17-aadA5-aadA4，dfrA1-aadA1-aadA2，dfrA1，dfrA5，aadA2 ，aacA4-catB8-aadA1-dfrA1-（aac6-II） - （blaCARB-8），blaPSE-1-blaP1）。最普遍的基因盒是dfrA17-aadA5（59.62％）。I类整合子阳性分离株对多种抗生素的抗性显着更高，并且它们通常对其I类整合子中含有的抗生素抗性基因盒展现相应的抗生素抗性谱。结果表明，Ⅰ型整合子与沙门氏菌分离株中普遍存在的不同抗生素抗性基因盒在省，市场和鸡储存条件方面不同。

Salmonella is one of the leading causes for foodborne diseases. Foods, particularly those of animal origin, act as an important role for Salmonella transmission. In this study, the antibiotic susceptibility of 743 Salmonella isolates recovered from retail raw chicken carcasses in eight provinces was tested, and the isolates were also screened for the presence of class I integron and drug-resistant gene cassettes. One hundred thirteen (15.21%) isolates were harboring class I integron. A higher percentage of integron-positive Salmonella isolates were found in retail chicken in Sichuan Province (29.33%), followed by Beijing (22.14%), Shaanxi (19.15%), Guangxi (14.13%), Henan (12.50%), Shanghai (7.25%), Fujian (8.22%), and Guangdong (6.25%) Provinces. The respective prevalence of class I integron in Salmonella isolates recovered from retail chickens in large, free, and small markets was 16.31%, 14.04%, and 15.27%. Moreover, 20.13%, 14.02%, and 13.74% ofSalmonella isolates recovered from retail chickens stored in frozen, chilled, and ambient conditions, respectively, were positive for class I integron. Subsequent sequencing of class I integron revealed the presence of 10 gene cassettes harboring resistance genes (dfrA17-aadA5, dfrA17-aadA5, dfrA1-aadA1, dfrA12-aadA2, dfrA17-aadA5-aadA4, dfrA1-aadA1-aadA2, dfrA1, dfrA5, aadA2, aacA4-catB8-aadA1-dfrA1-(aac6-II)-(blaCARB-8), blaPSE-1-blaP1). The most prevalent gene cassette was dfrA17-aadA5 (59.62%). Class I integron-positive isolates were significantly more resistant to multiple antibiotics, and they commonly exhibited corresponding antibiotic resistance profiles to the antibiotic resistance gene cassettes harbored in their class I integron. The results indicated that class I integron with different antibiotic resistance gene cassettes that were prevalent in Salmonella isolates differed from provinces, marketplaces, and chicken storage conditions.

https://www.liebertpub.com/doi/abs/10.1089/mdr.2015.0359