摘要

合成废水废水基质被设计成动力学上适当地反应真实样品，同时含有足够高浓度的抗生素红霉素用于微生物测定。在对金黄色葡萄球菌分离物进行初步实验之后，可以获得针对红霉素的耐药亚型和易感亚型。琼脂扩散和肉汤微量稀释测定表明抗生素处理的稀释水溶液中形成的产物的抗菌活性，但是，也显示了EB处理消除其活性的适用性。在复杂的废水基质中，尽管由基质组分（如腐殖酸，HCO3-）形成的二次自由基数量很高，但建议消除抗菌活性遵循关于与稀水溶液一致的抗生素的OH的化学计量。尽管会出现次要自由基有助于降解分子的情况，但根据最坏情况的方法，该技术需要根据化学计量的OH存在进行设计。

A synthetic effluent wastewater matrix was designed to be a kinetically appropriate reflection of a real sample while containing the antibiotic erythromycin at sufficiently high concentration for the microbiological assay. Following preliminary experiments on S. aureus isolates resistant and susceptible subtypes could be obtained against erythromycin. Agar diffusion and broth microdilution assays indicated antibacterial activity of the products forming in treated dilute aqueous solution of the antibiotic, however, also showed the applicability of EB treatment to eliminate their activity. In the complex wastewater matrix despite the high amount of secondary radicals forming from the matrix components (e.g. humic acid, HCO3−) the elimination of the antibacterial activity is suggested to follow the stoichiometry of OH in respect to the antibiotic in line with dilute aqueous solution. Although there will be cases when secondary radicals will help degrading a molecule, the technology needs to be designed to the stoichiometric presence of OH according to the worst-case scenario approach.

https://doi.org/10.1016/j.cej.2017.03.114