摘要

乳酸菌（LAB）菌株LAB-A，LAB-B和LAB-C从印度南部非常受欢迎的发酵食品中分离得到，并使用16S rRNA测序鉴定为物种水平，作为乳酸片球菌CSI29MX，小片状球菌MF 233和戊糖片球菌QN1D。所有三种菌株均产生抑制革兰氏阳性食源性病原体金黄色葡萄球菌和革兰氏阴性铜绿假单胞菌的细菌素。将LAB菌株包裹在海藻酸钙中以获得更高的细菌素生产。 72小时后，封装的LAB显示细菌素产量显着增加，分别针对金黄色葡萄球菌和铜绿假单胞菌分别测试2600和2800AU / ml，并且在24至72小时期间，封装的LAB的活细胞数量从4.5×10 6增加至6.3×10 6 / ml以分别释放1100AU / ml和1000AU / ml的细胞，对抗金黄色葡萄球菌和铜绿假单胞菌，并且在24小时后游离细胞从4.2×10 6至1.2×10 6 / ml显着降低。与1100和900AU / ml的游离细胞相比，包裹的嗜酸乳杆菌MTCC10307标准菌株表现出2000AU / ml和2200AU / ml。分离的乳酸片球菌CSI29MX，小片状小球菌MF 233和戊糖片球菌QN1D显示出比嗜酸乳杆菌MTCC 10307更高的细菌素生产潜力。

Lactic acid bacteria (LAB) strains LAB -A, LAB -B and LAB-C isolated from batter of idli, very popular fermented food of south India and identified to species level using 16 S rRNA sequencing as Pediococcus acidilactici CSI29MX, Pediococcus parvulus MF 233 and Pediococcus pentosaceus QN1D respectively. All the three strains produced bacteriocins that inhibited Gram positive food borne pathogen Staphylococcus aureus and Gram negative Pseudomonas aeruginosa. LAB strains were encapsulated in calcium alginate for possible higher bacteriocin production. After 72 h encapsulated LAB demonstrated remarkable increase in bacteriocin production with 2600 and 2800AU/ml tested against S.aureus and P.aeruginosa respectively and viable cell number of encapsulated LAB increased from 4.5 x106 to 6.3 x106/ml during 24 to 72 h as compared to free cells with 1100 AU/ml and 1000AU/ml, against S.aureus and P.aeruginosa respectively and decreased remarkably with free cells from 4.2 x106 to 1.2 x106/ml after 24 h. Encapsulated L. acidophilus MTCC 10307 , standard strain exhibited 2000 AU/ml and 2200 AU/ml compared to free cells with 1100 and 900 AU/ml. The isolates Pediococcus acidilactici CSI29MX, Pediococcus parvulus MF 233 and Pediococcus pentosaceus QN1D showed higher potential for bacteriocin production than L. acidophilus MTCC 10307.

http://oaji.net/articles/2017/736-1486989075.pdf