摘要

研究了产细菌素乳杆菌乳杆菌CRL1862及其细菌素在10°C工业表面对李斯特菌生物膜形成的控制作用。在不同的李斯特菌中进行筛选，允许选择单核细胞增生李斯特菌FBUTT作为其在不锈钢（SS）和聚四氟乙烯（PTFE）表面上的生物膜生产者。模拟了三种条件，以评价细菌素菌株移位、排除和竞争病原体生物膜形成的能力。SaCl乳杆菌CRL1862通过三种检测机制有效地抑制了单核细胞增生李斯特氏菌FBUTT的生物膜形成，在PTFE上的病原体抑制比SS表面更有效。此外，单核细胞增生李斯特菌FBUTT与细菌素产生菌的共培养效果最高，在PTFE和SS上分别降低了5.54±0.12和4.52±0.01。在工业上，用L.Saki-CCR1862或细菌素（排除）进行预处理是防止病原体生物膜沉降的最现实的方法。细菌素和/或细菌素产生菌菌株的使用是一种安全和环境友好的卫生方法，以减轻后处理食品污染。

The effect of the bacteriocin-producing Lactobacillus sakei CRL1862 and its bacteriocin in the control of Listeria biofilm formation on industrial surfaces at 10°C was investigated. A screening among different Listeriaspecies was performed allowing selecting L. monocytogenes FBUNT for its use as a biofilm producer on stainless steel (SS) and polytetrafluoroe-thylene (PTFE) surfaces. Three conditions were simulated to evaluate the ability of the bacteriocinogenic strain to displace, exclude and compete pathogen biofilm formation. Lactobacillus sakei CRL1862 effectively inhibited biofilm formation by L. monocytogenes FBUNT through the three assayed mechanisms, pathogen inhibition being more efficient on PTFE than on SS surface. Moreover, co-culture of L. monocytogenes FBUNT with the bacteriocin-producer displayed the highest efficacy reducing the pathogen by 5.54 ± 0.12 and 4.52 ± 0.01 on PTFE and SS, respectively. Industrially, the pre-treatment with L. sakeiCRL1862 or its bacteriocin (exclusion) constitutes the most realistic way to prevent pathogen biofilm settlement. The use of bacteriocins and/or the bacteriocin-producer strain represents a safe and environmentally-friendly sanitation method to mitigate post-processing food contamination.

https://academic.oup.com/femsle/article/363/12/fnw118/2570355