摘要

利用生物活性人工合成的两肽细菌素PlnEF研究其对敏感细菌植物乳杆菌pl2的作用方式。所获得的数据显示，PlnEF诱导膜透化，允许电解质流出，这表现在植物乳杆菌pl2细胞经PlnEF处理数分钟后，细胞外电导率增加、跨膜电势和pH梯度耗散以及细胞内ATP快速耗尽。激光共聚焦显微镜显示，PlnEF在植物乳杆菌pl2细胞中积累非常迅速，PlnEF的积累导致细胞膜损伤。扫描电子显微镜和透射电子显微镜进一步显示，PlnEF诱导植物乳杆菌pl2细胞发生形态变化和结构破坏，如气泡形成、微球形成、膜变形和细胞溶解。综上所述，所得数据显示PlnEF对植物乳杆菌pl2细胞造成细胞膜损伤。我们的研究揭示了双肽细菌素PlnEF对植物乳杆菌的抗菌机理。

Biologically active, artificially synthesized two-peptide bacteriocin PlnEF was used to study its mode of action on sensitive bacteria Lactobacillus plantarum pl2. The data obtained showed that PlnEF induced membrane permeabilization, allowing for the efflux of electrolytes, which was evidenced by the increased extracellular conductivity, the dissipation of transmembrane electrical potential and pH gradient, and rapid intracellular ATP depletion after L. plantarum pl2 cells were treated with PlnEF for minutes. Laser confocal microscopy showed that PlnEF accumulated very quickly in L. plantarum pl2 cells and the accumulation of PlnEF caused damage to cell membrane. Scanning electron microscopy and transmission electron microscopy further showed that PlnEF induced morphological changes and structure disruption to L. plantarum pl2 cells, such as the formation of blebs, microspheres, membrane deformation and cell lysis. In summary, the data obtained show that PlnEF caused cell membrane damage to L. plantarum pl2 cells. Our study reveals the antimicrobial mechanism of two-peptide bacteriocin PlnEF against L. plantarum.

https://www.sciencedirect.com/science/article/pii/S0005273615003909