摘要
革兰氏阳性乳酸菌（LAB）的成员因其作为发酵剂培养物和益生菌的有益特性而众所周知。许多LAB菌种产生核糖体合成的蛋白质抗生素（细菌素）。 Weissella confusa MBF8-1是一种从发酵大豆产品中分离得到的菌株，不仅产生有用的胞外多糖，而且还显示细菌素活性，我们称之为weissellicin MBF。在这里，我们显示由W.confusa MBF8-1产生的细菌素由大质粒pWcMBF8-1指定。从W.confusa MBF8-1草图基因组序列中鉴定的质粒pWcMBF8-1（GenBank登录号KR350502）长度为17643bp，G + C含量为34.8％，并含有25个开放阅读框（ORF） 。编码三个推定的双甘氨酸基序肽（Bac1，Bac2，Bac3），ABC转运蛋白复合物（BacTE）和推定的免疫蛋白（BacI）的六个ORF构成weissellicinMBF基因座。两个ORF编码质粒分配和动员蛋白，表明pWcMBF8-1可以转移到其他宿主。据我们所知，质粒pWcMBF8-1不仅代表要测序的第一个大型Weissella质粒，而且也是第一个与W. confusa中细菌素生产相关的质粒。

Members of the Gram-positive lactic acid bacteria (LAB) are well-known for their beneficial properties as starter cultures and probiotics. Many LAB species produce ribosomally synthesized proteinaceous antibiotics (bacteriocins). Weissella confusa MBF8-1 is a strain isolated from a fermented soybean product that not only produces useful exopolysaccharides but also exhibits bacteriocin activity, which we call weissellicin MBF. Here, we show that bacteriocin production by W. confusaMBF8-1 is specified by a large plasmid, pWcMBF8-1. Plasmid pWcMBF8-1 (GenBank accession number KR350502), which was identified from the W. confusa MBF8-1 draft genome sequence, is 17 643 bp in length with a G + C content of 34.8% and contains 25 open reading frames (ORFs). Six ORFs constitute the weissellicin MBF locus, encoding three putative double-glycine-motif peptides (Bac1, Bac2, Bac3), an ABC transporter complex (BacTE) and a putative immunity protein (BacI). Two ORFs encode plasmid partitioning and mobilization proteins, suggesting that pWcMBF8-1 is transferable to other hosts. To the best of our knowledge, plasmid pWcMBF8-1 not only represents the first large Weissella plasmid to be sequenced but also the first to be associated with bacteriocin production inW. confusa.

https://academic.oup.com/femsle/article/363/8/fnw059/2570135