摘要

侧孢霉灵10（LS10）是来自短芽孢杆菌属抑制微生物病原体的菌株SKDU10的防御素样肽。而且，在这项研究中，LS10的抗癌活性在不同的癌细胞系进行的，并与正常细胞进行比较。 低于10μM浓度的LS10对MCF-7，HEK293T，HT1080，HeLa和H1299等癌细胞显示出细胞毒性，但对前列腺上皮细胞RWPE-1无毒性。另外，与在不同的癌细胞系中观察到的IC 50值相比，在显着更高的浓度下没有观察到溶血。15μM浓度处理120分钟后从癌细胞系释放乳酸脱氢酶表明LS10的溶解能力。因此，电子显微镜实验也证实了15μM浓度的LS10对癌细胞的坏死作用。此外，处理的癌细胞系的流式细胞术分析揭示即使在2.5μM浓度下LS10也诱导细胞凋亡。尽管如此，甚至在20μM浓度下，RWPE-1细胞仍然存活。这些结果提供了LS10是抗癌细菌素的证据，其分别在较低和较高浓度下分别导致癌细胞的凋亡和坏死死亡。综合考虑所有结果，本研究表明LS10是一种抗癌肽，可进一步开发用于治疗应用。

Laterosporulin10 (LS10) is a defensin like peptide from Brevibacillus sp. strain SKDU10 that inhibited microbial pathogens. However, in this study, anticancer activity of LS10 was examined against different cancer cell lines and compared with normal cells. LS10 displayed cytotoxicity against cancer cells like MCF-7, HEK293T, HT1080, HeLa and H1299 at below 10 μM concentration, but not against prostate epithelium cells RWPE-1. Additionally, no hemolysis was observed at significantly higher concentration compared to IC50 values observed for different cancer cell lines. Release of lactate dehydrogenase from cancer cell lines at 15 μM concentration upon 120 min treatment indicated the lytic ability of LS10. Accordingly, electron microscopy experiments also confirmed the necrotic effect of LS10 at 15 μM concentration against cancer cells. Furthermore, flow cytometry analysis of treated cancer cell lines revealed that LS10 induce apoptosis even at 2.5 μM concentration. Nevertheless, RWPE-1 cells remained viable even at 20 μM concentration. These results provide evidence that LS10 is an anticancer bacteriocin, which causes apoptotic and necrotic death of cancer cells at lower and higher concentrations, respectively. Taken all results together, the present study signifies that LS10 is an anticancer peptide that could be further developed for therapeutic applications.

https://www.nature.com/articles/srep46541