以乳清为底物的乳酸双歧杆菌产生细菌素类抑制物质（BLIS）
发布者：抗性基因网 时间：2018-06-27 浏览量：1203
摘要
这项工作的目的是评估由动物双歧杆菌亚种产生细菌素类抑制物质（BLIS）。 添加了酵母提取物，菊糖，吐温-80或L-半胱氨酸的乳清中的乳清。 在摇瓶中进行发酵期间测量细胞生长，酸化，葡萄糖和乳糖消耗以及BLIS产生。 表明细胞生长和BLIS产生的最佳添加剂是酵母提取物，其提供最高浓度的生物量（9.9log cfu / mL）和BLIS（800AU / mL）。 在台式发酵罐中，取决于测定的条件，乳酸乳球菌生长和BLIS产量比烧瓶高6％至25％。
The objective of this work was to evaluate the production of bacteriocin‐like inhibitory substances (BLIS) by Bifidobacterium animalis subsp. lactis in whey supplemented with yeast extract, inulin, Tween‐80 or l‐cysteine. Cell growth, acidification, glucose and lactose consumption as well as BLIS production were measured during fermentations carried out in shake flasks. The best additive for both cell growth and BLIS production was shown to be yeast extract, which gave the highest concentrations of biomass (9.9 log cfu/mL) and BLIS (800 AU/mL). In a bench‐scale fermentor, B. lactis growth and BLIS production were between 6% and 25% higher than in flasks depending on the conditions assayed.
https://onlinelibrary.wiley.com/doi/full/10.1111/1471-0307.12247
