摘要
本研究的目的是通过抑制痤疮丙酸杆菌诱导的白细胞介素-8（IL-8）释放来评估唾液乳杆菌LS03（DSM 22776）对痤疮丙酸杆菌的抗菌活性及其抗炎特性.痤疮是最常见的皮肤病，对受折磨的人造成重大社会心理问题。目前可用于痤疮治疗的药剂，例如口服抗生素，使用有限。因此，需要开发治疗该疾病的新药。在炎性病变的产生中，阻塞性卵泡中痤疮丙酸杆菌的增殖是至关重要的。有益微生物的施用代表了治疗多种皮肤改变的有希望的方法并且可以具有许多有利效果。对于抑制测定，将痤疮丙酸杆菌涂布在丙酸杆菌分离琼脂基板上，并将LS03浸泡的盘直接置于琼脂表面上。将从健康志愿者分离的外周血单核细胞与植物血凝素1μg/ mL预孵育1小时，并用益生菌菌株刺激24小时以模拟体外IL-8释放模型。使用ELISA试剂盒，一式两份分析上清液中的IL-8浓度。唾液乳杆菌LS03对靶病原体菌株发挥显着的抑制能力。该拮抗活性主要归因于分泌针对痤疮丙酸杆菌的活性细菌素的LS03菌株的特征。关于IL-8分析，3种不同的唾液乳杆菌菌株能够抑制该趋化因子的释放10％至25％。唾液LS03益生菌菌株可能是寻常痤疮患者抗生素/抗炎治疗的替代疗法。

The aim of this research was to assess the antibacterial activity of Lactobacillus salivarius LS03 (DSM 22776) against Propionibacterium acnes and its anti-inflammatory properties by inhibiting P. acnes-induced interleukin-8 (IL-8) release.Acne is the most common skin disease, causing significant psychosocial problems for those afflicted. Currently available agents for acne treatment, such as oral antibiotics, have limited use. Thus, development of novel agents to treat this disease is needed. In the generation of inflammatory lesions, proliferation of P. acnes in the obstructed follicles is critical. The administration of beneficial microorganisms represents a promising approach for treating several skin alterations and can have many favorable effects.For the inhibition assay, P. acnes was spread on Propionibacter Isolation Agar Base plates, and LS03-soaked disks were placed directly on the agar surface. Peripheral blood mononuclear cells, isolated from healthy volunteers, were preincubated with phytohemagglutinin 1 μg/mL for 1 hour and stimulated with the probiotic strains for 24 hours to simulate an in vitro IL-8 release model. The IL-8 concentration in the supernatants was analyzed in duplicate using ELISA Kit.L. salivarius LS03 exerted a significant inhibitory capacity against the target pathogen strain. This antagonistic activity was primarily ascribable to the feature of LS03 strain of secreting active bacteriocins against P. acnes. Concerning the IL-8 analysis, 3 different L. salivarius strains were able to inhibit the release of this chemokine by 10% to 25%.L. salivarius LS03 probiotic strain could be an alternative treatment to antibiotic/anti-inflammatory therapy in subjects presenting acne vulgaris.
https://europepmc.org/abstract/med/29782471