摘要

从动物及其环境中传播的抗生素耐药性是一个公共问题，对人类健康构成重大威胁。在本研究中，对来自虾的内脏和相关水产养殖环境（水和沉积物）的15个样品中的抗生素抗性基因（ARG）的多样性和丰度进行了研究。在虾肠，池塘水和沉积物样品中共分别检测到60种ARG，102种ARG和67种ARG，主要分别为13种，15种和15种不同类型。在所有样品中，外排泵和靶标修饰是主要的抗性机制。结果发现，气单胞菌，耶尔森氏菌和Clostridium XlVb与ARG的分布显着相关。此外，ARG的相对丰度与移动遗传元件（MGEs）的水平正相关。此外，变异划分分析表明，MGEs在抗生素抗性的影响中起着重要作用，在抗生素抗性组织中的作用比细菌群落及其联合作用更为重要。总的来说，这项研究提供了全面的信息，以便更好地了解水产养殖环境中的ARG传播并改善水生生态系统的生态管理。

Antibiotic resistance disseminating from animals and their environments is a public issue that poses significant threats to human health. In the present study, the diversity and abundance of antibiotic resistance genes (ARGs) in 15 samples from the guts and related aquaculture environments (water and sediment) of shrimp were in-vestigated. In total, 60 ARGs, 102 ARGs and 67 ARGs primarily belonging to 13, 15 and 15 different types were detected in the shrimp gut, pond water and sediment samples, respectively. Efflux pump and target modification were the predominant resistance mechanisms in all samples. It was found that Aeromonas, Yersinia and Clostridium XlVb were significantly correlated with the distribution of the ARGs. Besides, the relative abundance of ARGs was positively correlated with the levels of mobile genetic elements (MGEs). Moreover, variation partitioning analysis showed that MGEs, contributing to 74.46% of the resistome variation, played an important role in the affecting of the antibiotic resistome than the bacterial communities and their joint effects. Collectively, this study provides comprehensive information to better understand the ARG dissemination in aquaculture environments and to improve the ecological management of aquatic ecosystems.
https://www.sciencedirect.com/science/article/pii/S0304389418300803