摘要

这项横断面研究于2015年1月至2015年12月在孟加拉国达卡达卡医学院微生物学系进行，旨在研究抗生素耐药模式和编码氨基糖苷类修饰酶的基因在大肠杆菌和克雷伯氏菌中的流行情况。孟加拉国的三级医院。从达卡医学院附属医院患者的尿液，伤口拭子，脓液，痰液和血液样本中分离出166株大肠埃希氏菌，肺炎克雷伯菌和产气克雷伯菌。通过盘扩散技术进行抗生素敏感性试验。通过聚合酶检测分离的大肠杆菌和克雷伯氏菌中氨基糖苷类修饰酶[aac（3）-IIa，aac（6'） - Ib，ant（2“） - Ia，aac（3）-Ia]的基因连锁反应。发现最常见的氨基糖苷类修饰酶基因是aac（3）-IIa（60.24％），其次是aac（6'） - Ib（57.23％），ant（2''） - Ia（12.05％）和aac（ 3）-Ia（10.84％）。粘菌素和替加环素是最有效的抗氨基糖苷类修饰酶的抗菌剂，其次是亚胺培南。该研究的结果显示，在孟加拉国，高比例的氨基糖苷类修饰酶产生大肠杆菌和克雷伯氏菌。

This cross-sectional study was conducted in the Department of Microbiology of Dhaka Medical College, Dhaka, Bangladesh, from January 2015 to December 2015 to investigate the antibiotic resistance pattern and prevalence of genes encoding aminoglycoside-modifying enzymes in Escherichia coli and Klebsiella species isolated from a tertiary care hospital of Bangladesh. A total of 166 Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca were isolated from urine, wound swab, pus, sputum and blood samples of patients of Dhaka Medical College Hospital. Antibiotic susceptibility test was performed by disk-diffusion technique. Genes encoding aminoglycoside-modifying enzymes [aac(3)-IIa, aac(6')-Ib, ant(2'')-Ia, aac(3)-Ia] among the isolated Escherichia coli and Klebsiella species were detected by polymerase chain reaction. The most prevalent aminoglycoside-modifying enzyme gene found was aac (3)-IIa (60.24%), followed by aac(6')-Ib (57.23%), ant(2'')-Ia (12.05%) and aac(3)-Ia (10.84%). Colistin and tigecycline were the most effective antibacterial agents against aminoglycoside-modifying enzymes followed by imipenem. The results of this study showed high proportion of aminoglycoside-modifying enzymes producing Escherichia coli and Klebsiella species in Bangladesh.

https://www.ncbi.nlm.nih.gov/pubmed/30141446