摘要

牛乳腺炎给奶农带来了严重的经济损失。金黄色葡萄球菌（Staphylococcus aureus）是牛在临床和亚临床乳腺炎病因学中最重要的病原体之一。鉴于抗菌药物耐药性增加，非常需要替代抗生素治疗。目前的十年见证了对基于噬菌体的治疗和诊断的新兴趣。本研究描述了两种裂解噬菌体SAJK-IND和MSP对金黄色葡萄球菌的分离和表征，该金黄色葡萄球菌有可能用于治疗乳腺炎。 SAJK-IND和MSP噬菌体分别属于Myoviridae和Podoviridae科。两个噬菌体的TEM成像显示出一个等粘性的头部。 MSP噬菌体具有短的不可收缩的尾巴。 SAJK-IND和MSP的突发大小分别为44±3和25±5PFU /感染细胞。 SAJK-IND和MSP噬菌体分别在SDS-PAGE分析中显示̴12和̴16蛋白。噬菌体的裂解活性对金黄色葡萄球菌是特异性的。 SAJK-IND显示对从乳腺炎乳样品中分离的几种金黄色葡萄球菌菌株具有100％的裂解活性，而MSP仅具有40％的裂解活性。对SAJK-IND噬菌体基因组进行测序，组装并以登录号MG010123保藏在Genbank中。

Bovine mastitis causes severe economic losses to dairy farmers. Staphylococcus aureus, is one of the most important pathogen implicated in etiology of clinical and subclinical mastitis in bovines. In view of increasing antimicrobial resistance alternatives to antibiotic therapy are much needed. The present decade has witnessed a renewed interest in phage based therapeutics and diagnostics. The present study, describes isolation and characterization of two lytic phages SAJK-IND and MSP against Staphylococcus aureus having a potential to be used in therapy against mastitis. SAJK-IND and MSP phages belonged to Myoviridae and Podoviridae families, respectively. TEM imaging of the two phages revealed an iscosahedral head. MSP phage has a short non contractile tail. SAJK-IND and MSP have a burst size of 44 ± 3 and 25 ± 5 PFU/ infected cell, respectively. SAJK-IND and MSP phages revealed ̴ 12 and ̴16 proteins, respectively on SDS-PAGE analysis. The lytic activity of the phages was specific for Staphylococcus aureus. SAJK-IND revealed 100% lytic activity against several strains of Staphylococcus aureus isolated from mastitis milk samples whereas, MSP had only 40% lytic activity. SAJK-IND phage genome was sequenced, assembled and deposited in Genbank under accession no MG010123.

https://www.ncbi.nlm.nih.gov/pubmed/30219981