摘要

本文研究了10个碳青霉烯酶基因，并在渤海湾河口选择了这些基因的宿主。结果显示，OXA-58生产者在采样点中占很大比例的碳青霉烯抗性细菌，而VIM，KPC，NDM，IMP，GES，OXA-23，OXA-24，OXA-48和OXA-51研究中未检测到生产者。此外，从海水中分离出9种具有100％相同的blaOXA-58序列的细菌属，包括假单胞菌，Rheinheimera，Stenotrophomonas，Shewanella，Raoultella，Vibrio，Pseudoalteromonas，Algoriphagus，Bowmanella和Thalassospira。提示blaOXA-58基因的宿主是多样的，并且它们中的许多可以在海水中存活。此外，我们进行了定量RT-PCR，结果显示blaOXA-58的丰度在2.8×10-6拷贝/16S和2.46×10-4拷贝/ 16S之间波动，这与一些常见的数量级相同。环境中的抗生素抗性基因。此外，blaOXA-58基因的变异趋势表明污染排放和水平基因转移可能有助于沿海地区基因的增加。

This paper investigated 10 carbapenemase genes and selected the hosts of these genes in the estuary of Bohai Bay. The results showed that the OXA-58 producer accounted for a large percentage of carbapenem resistant bacteria in the sampling points, whereas the VIM, KPC, NDM, IMP, GES, OXA-23, OXA-24, OXA-48 and OXA-51 producers were not detected in the study. In addition, 9 bacterial genera with 100% identical blaOXA-58 sequences, including Pseudomonas, Rheinheimera, Stenotrophomonas, Shewanella, Raoultella, Vibrio, Pseudoalteromonas, Algoriphagus, Bowmanella and Thalassospira, were isolated from seawater. It is suggested that the host of blaOXA-58 gene were varied and many kinds of them could survive in the seawater. Moreover, we preformed the quantitative RT-PCR and the result shown the abundance of blaOXA-58 fluctuated between 2.8×10-6 copies/16S and 2.46×10-4 copies/16S, which was of the same order of magnitude as some common antibiotic resistance genes in environment. Furthermore, the variation trend of blaOXA-58 gene suggested that pollution discharge and horizontal gene transfer could contribute to the increase of the gene in coastal area.

https://www.ncbi.nlm.nih.gov/pubmed/30321713