背景：
Integron，ISCR1和复杂的1类整合子使细菌对抗生素方案产生抗性。本研究的目的是检测多药耐药革兰阴性杆菌的常见移动遗传因素，并评估中国南方败血症患者血液标本中这些杆菌的基因分型。

方法：
在中国南方2011年至2014年间从败血症患者采集的血液样本中，共研究了837种革兰阴性杆菌，包括578株含有肠杆菌科的菌株和259株含有非发酵杆菌的菌株。从837株中检测到移动遗传元件，例如1类整合子，插入序列共同区域1（ISCR1）和复合物1类整合子。

结果：
在837个菌株中发现了27种基因盒阵列，其中发现492（58.8％）1类整合子阳性分离株和254（51.6％）基因盒阳性分离株，包括两种类型的第一种描述，aacA4- bla IMP-1-bla OXA-30-catB3和aac（6'） - II-aadA13-cmlA8-bla OXA-10，在相应的物种和两个基因盒中，推定的解旋酶和aadA样，最初在整合子中检测到。获得了196种ISCR1阳性杆菌中的12种ISCR1连接的抗性基因区域和7种不同类型的复合物1型整合子阳性菌株，包括从未在任何物种中描述的四种不同的复合物1类整合子。肠杆菌重复基因间共有（ERIC）-PCR fngerprinting显示具有相同基因谱的分离株在克隆上无关。

结论：
我们的研究结果表明，我们应该更加注重提高感染控制措施的质量，防止医院感染，以避免多重耐药革兰阴性杆菌的爆发。

BACKGROUND:
Integron, ISCR1 and complex class 1 integrons lead bacteria to become resistant to antibiotic regimens. The aim of this study was to detect common mobile genetic elements of multidrug-resistant Gram-negative bacilli and evaluate the genotyping of these bacilli in blood specimens from septicemia patients in southern China.

METHODS:
A total of 837 Gram-negative bacilli including 578 strains containing Enterobacteriaceae and 259 strains containing non-fermentative bacilli were investigated in blood samples collected from septicemia patients between 2011 and 2014 in southern China. Mobile genetic elements, such as class 1 integrons, the insertion sequence common region 1 (ISCR1), and complex class 1 integrons, were detected from the 837 strains.

RESULTS:
Twenty-seven types of gene cassette arrays were found among 837 strains in which 492 (58.8%) class 1 integron-positive isolates and 254 (51.6%) gene cassette-positive isolates were found, including the first description of two types, aacA4-bla IMP-1-bla OXA-30-catB3 and aac(6')-II-aadA13-cmlA8-bla OXA-10, in the corresponding species and two gene cassettes, putative helicase and aadA-like, originally detected in integrons. Twelve types of ISCR1-linked resistance gene regions in 196 ISCR1-positive bacilli and seven different types of complex class 1 integron-positive strains were obtained including four distinct complex class 1 integrons that have never been described in any species. Enterobacterial repetitive intergenic consensus (ERIC)-PCR fngerprinting showed that isolates with identical gene profles were clonally unrelated.

CONCLUSION:
Our results indicated that we should pay more attention to enhance the quality of infection control measures and prevent hospital infection, so as to avoid the outbreak of multidrug-resistant Gram-negative bacilli.

https://www.ncbi.nlm.nih.gov/pubmed/30349331