摘要

倍半萜内酯（Heptelidic acid，HA）是已知的甘油醛-3-磷酸脱氢酶（GAPDH）抑制剂。 最近，我们发现HA是由米曲霉RIB40产生的，并且在酱油酿造中充当耐盐乳酸菌的生长抑制剂。 尽管自发现HA已经过去了几十年，但其生物合成和生物合成途径中涉及的基因尚未完全确定。 在这项研究中，我们使用基因破坏和表达分析鉴定了HA生物合成基因簇（HA簇）。 我们还揭示了与HA簇相邻的两个转录调节基因负责米曲霉中HA生物合成基因的表达。 有趣的是，HA簇包含编码GAPDH（gpdB）的基因，其显示出比位于其他基因座的GAPDH基因（gpdA）高得多的HA抗性，但其似乎不是自身抗性基因。

Heptelidic acid (HA), a sesquiterpene lactone, is a known inhibitor of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Recently, we found that HA was produced by Aspergillus oryzae RIB40 and acted as the growth inhibitor of the salt-tolerant lactic acid bacteria in soy sauce brewing. Although several decades have passed since the discovery of HA, the genes involved in its biosynthesis and biosynthetic pathway have not yet been fully identified. In this study, we identified the HA biosynthetic gene cluster (HA cluster) using gene disruption and expression analysis. We also revealed that two transcription regulatory genes adjacent to the HA cluster were responsible for the expression of HA biosynthetic genes in A. oryzae. Interestingly, the HA cluster contained a gene encoding GAPDH (gpdB), which showed much higher resistance to HA than the GAPDH gene (gpdA) located at the other locus, but which did not seem to act as a self-resistant gene.

https://www.ncbi.nlm.nih.gov/pubmed/30466366