摘要

由于其抗生素耐药性，多重耐药性肠球菌是医疗相关感染的主要原因。其中，粪肠球菌是一种重要的机会致病菌，可引起各种医院获得性感染。从各种感染中获得总共53种粪肠球菌分离物。它们通过表型和基因型方法鉴定。根据CLSI指南确定抗微生物剂抗性模式。对≥3种抗微生物类别中至少一种药剂不敏感的菌株被定义为多药耐药（MDR）。使用标准程序进行抗微生物抗性基因的检测。根据MDR定义，所有分离株均为MDR（100％）。在其中50.9％（MIC≥500μg/ ml）中观察到高水平的庆大霉素抗性。 aac（6'） - Ie-aph（2''） - Ia和aph（3'） - IIIa基因的分布分别为47.2％和69.8％。未检测到aph（2''） - Ib，aph（2“） - Ic，aph（2”） - Id和蚂蚁（4'） - Ia基因。在45.3％的菌株中发现了万古霉素抗性。在37.7％的分离株中检测到vanA基因，而在任何菌株中均未观察到vanB和vanC1基因。红霉素耐药率为79.2％，ermB和ermC基因频率分别为88.6％和69.8％。 ermA和msrA基因不存在于任何分离株中。我们的数据表明MDR粪肠球菌菌株的比例很高。所有高水平的庆大霉素抗性分离株携带至少一种aac（6'） - Ie-aph（2''） - Ia或aph（3'） - IIIa基因。 vanA的分布在分离株中是显着的。此外，ermB和ermC对红霉素的抗性负责。

Multiple drug-resistant enterococci are major cause of healthcare-associated infections due to their antibiotic resistance traits. Among them, Enterococcus faecalis is an important opportunistic pathogen causing various hospital-acquired infections. A total of 53 E. faecalis isolates were obtained from various infections. They were identified by phenotypic and genotypic methods. Determination of antimicrobial resistance patterns was done according to CLSI guidelines. The isolates that were non-susceptible to at least one agent in ≥3 antimicrobial categories were defined as multidrug-resistant (MDR). Detection of antimicrobial resistance genes was performed using standard procedures. According to MDR definition, all of the isolates were MDR (100%). High-level gentamicin resistance was observed among 50.9% of them (MIC ≥ 500 μg/ml). The distributions of aac(6')-Ie-aph(2'')-Ia and aph(3')-IIIa genes were 47.2% and 69.8%, respectively. The aph(2'')-Ib, aph(2'')-Ic, aph(2'')-Id, and ant(4')-Ia genes were not detected. Vancomycin resistance was found in 45.3% of strains. The vanA gene was detected in 37.7% of isolates, whereas vanB and vanC1 genes were not observed in any strain. Erythromycin resistance rate was 79.2% and the frequencies of ermB and ermC genes were 88.6% and 69.8%, respectively. The ermA and msrA genes were not present in any of the isolates. Our data indicate a high rate of MDR E. faecalis strains. All of high-level gentamicin-resistant isolates carried at least one of aac(6')-Ie-aph(2'')-Ia or aph(3')-IIIa genes. Distribution of vanA was notable among the isolates. In addition, ermB and ermC were accountable for resistance to erythromycin.

https://www.ncbi.nlm.nih.gov/pubmed/30465449