摘要

为了阻止多重耐药细菌的上升趋势，研究人员转向利基环境，希望发现新的抗生素品种。我们在Fermanagh Scarplands（N。Ireland）的波西米亚地区的碱性/氡土壤中研究了一种民族药理学（治愈），因为链霉菌是一种着名的抗生素生产商。从这个土壤中我们分离出一种新的（最接近57％的基因组相关性）Streptomyces sp。能够在高碱性pH（10.5）下生长并且能够耐受γ射线到4kGy。与天蓝色链霉菌M145（3：1）相比，基因组测序鉴定了许多碱性耐受性（逆向转运蛋白/多抗性）基因，因此我们将菌株Streptomyces sp。 myrophorea，隔离McG1，来自希腊语，myro（香水）和phorea（搬运工/运营商）。体外试验证明了链霉菌属的能力。 myrophorea，分离McG1以抑制许多ESKAPE病原体的生长;最值得注意的是碳青霉烯类耐药鲍曼不动杆菌（世界卫生组织抗生素耐药细菌优先列表中的关键病原体），耐万古霉素屎肠球菌和耐甲氧西林金黄色葡萄球菌（均列为高优先级病原体）。进一步计算机预测链霉菌属的抗菌潜力。 myrophorea，通过抗SMASH分离McG1和RAST软件鉴定了许多次级代谢产物和毒性抗性基因簇（分别为45和27）以及许多可能与抗生素产生相关的抗生素抗性基因。随访体外试验表明，Streptomyces sp。 myrophorea，分离McG1对36种临床抗生素中的28种具有抗性。虽然不是一个全面的分析，但我们认为一些波西米亚土壤的知名治疗特性可能与链霉菌属的能力有关。 myrophorea，分离McG1以抑制ESKAPE病原体。更重要的是，进一步的分析可能会阐明可以缓解多重耐药性医院感染潮流的其他关键因素。

In an effort to stem the rising tide of multi-resistant bacteria, researchers have turned to niche environments in the hope of discovering new varieties of antibiotics. We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces, a well-known producer of antibiotics. From this soil we isolated a novel (closest relative 57% of genome relatedness) Streptomyces sp. capable of growth at high alkaline pH (10.5) and tolerant of gamma radiation to 4 kGy. Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. myrophorea, isolate McG1, from the Greek, myro (fragrance) and phorea (porter/carrier). In vitro tests demonstrated the ability of the Streptomyces sp. myrophorea, isolate McG1 to inhibit the growth of many strains of ESKAPE pathogens; most notably carbapenem-resistant Acinetobacter baumannii (a critical pathogen on the WHO priority list of antibiotic-resistant bacteria), vancomycin-resistant Enterococcus faecium, and methicillin-resistant Staphylococcus aureus (both listed as high priority pathogens). Further in silico prediction of antimicrobial potential of Streptomyces sp. myrophorea, isolate McG1 by anti-SMASH and RAST software identified many secondary metabolite and toxicity resistance gene clusters (45 and 27, respectively) as well as many antibiotic resistance genes potentially related to antibiotic production. Follow-up in vitro tests show that the Streptomyces sp. myrophorea, isolate McG1 was resistant to 28 out of 36 clinical antibiotics. Although not a comprehensive analysis, we think that some of the Boho soils' reputed curative properties may be linked to the ability of Streptomyces sp. myrophorea, isolate McG1 to inhibit ESKAPE pathogens. More importantly, further analysis may elucidate other key components that could alleviate the tide of multi-resistant nosocomial infections.

https://www.ncbi.nlm.nih.gov/pubmed/30459722