摘要

本文的目的是描述：春季波兰南部办公楼中可培养细菌气溶胶（QCBA）的数量和可培养细菌气溶胶（QlCBA）的质量。该建筑物中可培养细菌气溶胶（CCBA）的平均浓度范围为424 CFU m-3至821 CFU m-3，低于波兰关于阈值极限值的建议。尺寸分布是单峰的，颗粒细菌空气动力学直径的峰值小于3.3μm，由于它们的吸入而增加潜在的不利健康影响。估计细菌气溶胶的弹簧局暴露剂量（SPED）。 SPED的最高值是4月份（218 CFU kg-1），而最低值是6月份（113 CFU kg-1）。进行分析以确定分离的菌株的抗生素抗性及其形成生物膜的能力，这可能促进抗生素抗性基因的扩散。在研究过程中，发现木葡萄球菌具有最强的形成生物膜的能力，而具有最高抗生素抗性的菌株是藤黄微球菌D和大马尾松（Macrococcus equipercicus）。鉴于主要从生物气溶胶中分离抗生素敏感细菌，将抗性基因转移到质粒，这表明需要加强对工作场所室内空气质量的监测。

The aims of this article are to characterize: the quantity of culturable bacterial aerosol (QCBA) and the quality of culturable bacterial aerosol (QlCBA) in an office building in Southern Poland during the spring. The average concentration of culturable bacterial aerosol (CCBA) in this building ranged from 424 CFU m-3 to 821 CFU m-3, below Polish proposals for threshold limit values. Size distributions were unimodal, with a peak of particle bacterial aerodynamic diameters less than 3.3 μm, increasing potentially adverse health effects due to their inhalation. The spring office exposure dose (SPED) of bacterial aerosol was estimated. The highest value of SPED was in April (218 CFU kg-1), whereas the lowest was in June (113 CFU kg-1). Analysis was undertaken to determine the antibiotic resistance of isolated strains and their ability to form biofilms, which may facilitate the spread of antibiotic resistance genes. In the course of the study, it was found that Staphylococcus xylosus had the greatest ability to form biofilms, while the strains with the highest antibiotic resistance were Micrococcus luteus D and Macrococcus equipercicus. Given that mainly antibiotic-sensitive bacteria from bioaerosol were isolated, which transfers resistance genes to their plasmids, this shows the need for increased monitoring of indoor air quality in workplaces.

https://www.ncbi.nlm.nih.gov/pubmed/30469413