摘要

本研究旨在研究埃及肉鸡中分离出的沙门氏菌对某些抗菌药物的耐药性。还仔细检查了回收的多药耐药（MDR）分离株中一些毒力和抗性基因的流行程度。从353种不同的样本（肝脏，卵黄囊，胆囊和盲肠）中回收了总共55种（15.6％）沙门氏菌分离物，这些样本来自表现为腹泻，脱水，呼吸窘迫的健康和患病的肉鸡。将三十种沙门氏菌菌株血清分型为肠炎沙门氏菌，S.Infantis，S.Kn Kentucky，S.Maloma，S.Bardo，S.Gdansk，S.Typhimurium和S.Blegdame。构建了所有沙门氏菌分离株的抗性模式，随后检测了15株MDR沙门氏菌分离株的毒力[invA，ompA和stn]和抗性[qnrS，qnrA，blaTEM和blaCTX]基因的存在。值得注意的是，在所有检测的分离株中都发现了invA，ompA和stn毒力基因以及blaTEM和qnrS抗性基因。另一方面，qnrA基因检测频率为20％，而根本未检测到blaCTX。我们的研究结果强调了抗菌素耐药基因在沙门氏菌分离株中的广泛传播以及该疾病有效控制措施的重要性。

The present study was carried out to investigate the resistance to some antimicrobial groups among Salmonellae isolated from broilers in Egypt. The prevalence of some virulence and resistance genes among the recovered multidrug resistant (MDR) isolates was also scrutinized. A total of 55 (15.6%) Salmonella isolates were recovered from 353 different samples (liver, yolk sac, gall bladder, and caecum), gathered from apparently healthy and diseased broilers suffered from diarrhea, dehydration, respiratory distress. Thirty Salmonellae strains were serotyped into S. Enteritidis, S. Infantis, S. Kentucky, S. Maloma, S. Bardo, S. Gdansk, S. Typhimurium and S. Blegdame. The resistance pattern of all Salmonella isolates was constructed and fifteen MDR Salmonella isolates were subsequently examined for the presence of virulence [invA, ompA and stn] and resistance [qnrS, qnrA, blaTEM and blaCTX ] genes. Of note, invA, ompA and stn virulence genes and blaTEM and qnrS resistance genes were found in all examined isolates. On the other hand, the qnrA gene detection frequency was 20%, whereas blaCTX was not detected at all. Our findings emphasize the wide spread of antimicrobial resistance genes in Salmonella isolates and the importance of effective control measures for the disease. 

https://www.ncbi.nlm.nih.gov/pubmed/30561005