摘要

本研究建立了两个嗜温厌氧消化池，以确定抗生素抗性基因（ARGs）在高浓度抗生素污泥厌氧消化（AD）过程中的微生物动态和变异特征。分析了系统参数，微生物群落，ARG（tetA，tetM，tetW，sulI，sulII）和1类整合酶基因（intI1）。 AD的一般表现表明，在抗生素的压力下甲烷产量被抑制了17.1％。微生物16S rRNA高通量测序结果显示微生物群落丰富度下降，但添加抗生素的多样性更高。此外，属的水平微生物群落结构发生了显着变化。几种靶基因的实时定量PCR表明，高浓度抗生素的作用对ARGs产生了显着的诱导影响，但intI1的丰度明显下降。相关分析显示，intI1在AD期间仅在ARGs转移中起很小作用，潜在宿主的变化是关键因素。

This study established two mesophilic anaerobic digesters to ascertain the microbial dynamics and variation characteristics of antibiotic resistance genes (ARGs) during sludge anaerobic digestion (AD) with high concentration of antibiotics. System parameters, microbial community, ARGs (tetA, tetM, tetW, sulI, sulII) and integrase gene of class 1 (intI1) were analyzed. General performance of AD showed methane production was inhibited by 17.1% under the pressure of antibiotics. Microbial 16S rRNA high-throughput sequencing results showed the richness of microbial community decreased, but a higher diversity was found with antibiotics added. Furthermore, microbial community structure at genus level was significantly changed. Real-time quantitative PCR of several target genes demonstrated that the adjunction of high concentration of antibiotics exerted a significant induction influence on ARGs, however, the abundance of intI1 decreased observably. Correlation analysis showed intI1 only played a small role in ARGs' transfer during AD, change of potential hosts was the key factor instead.

https://www.ncbi.nlm.nih.gov/pubmed/30611086