摘要

  近年来，抗生素抗性基因（ARGs）作为新出现的环境污染物引起了许多关注。目前，抗生素对环境污染的研究主要集中在医药，畜牧业和水产养殖领域，很少有关于农业抗生素在植物保护领域的环境污染研究。武夷菌素是一种低毒，高效，广谱的农业抗生素。它已广泛用于农业生产，并有效控制作物真菌病害。在本研究中，在温室中建立了四种土壤处理（A，B，C和D）的盆栽试验，以研究武夷菌素应用对典型ARGs和微生物群落命运的影响。通过实时PCR检测到8种典型的ARGs，并使用高通量测序分析微生物群落。结果表明，武夷菌素的处理后既不显着影响ARGs丰度和基因绝对拷贝数，也不显着改变处理间的微生物群落。由于只是短期结果，并且ARGs的检测数量有限，因此在长期使用时，武夷菌素对生态环境是否安全还需要进一步深入研究。

  Abstract

  Antibiotic resistance genes (ARGs) have raised numerous concerns in recent years as emerging environmental contaminants. At present, research on environmental contamination by antibiotics focuses on medical, animal husbandry, and aquaculture fields, with few studies on environmental contamination by agricultural antibiotics in the field of plant protection. Wuyiencin is a low toxicity, high efficiency, and broad-spectrum agricultural antibiotic. It has been widely used in agricultural production and it effectively controls crop fungal diseases. In the present study, pot experiments with four soil treatments (A, B, C and D) were set up in a greenhouse to investigate the effect of the application of wuyiencin on the fate of typical ARGs and microbial community. Eight typical ARGs were detected by real-time PCR and themicrobial communities were analyzed using high-throughput sequencing. The results showed that wuyiencin neither significantly influenced ARG abundance and absolute gene copy numbers, nor significantly varied microbial community among treatments. Since it only was short-term results, and the detection number of ARGs was limited, whether wuyiencin is safe or not to ecological environment when using for long-term will need further deep research.