摘要

对抗生素的耐药性有可能成为全世界的健康问题。在这种情况下，一个重要的归因现象是病原体可以通过质粒的结合转移获得抗生素抗性基因。为了防止在农业环境中的细菌感染，兽医卫生产品的使用，例如消毒剂，已经普及，并且人们质疑它们对这种抗生素耐药性传播的贡献。因此，本研究调查了亚抑制浓度的季铵盐化合物（QAC）苯扎氯铵（BKC）对抗生素抗性基因的共轭转移的影响。将五个来自肉鸡的大肠杆菌野外菌株暴露于抑制性最低的BKC浓度：最低杀菌浓度的1 / 3、1 / 10和1/30，这些菌株来自已知的可转移质粒介导的环丙沙星抗性。使用大肠杆菌K12 MG1655作为受体菌株，通过在25°C液体交配4小时来评估抗生素耐药性转移。转移率的计算方法是转结合物的数量除以受体的数量。在不暴露于BKC的情况下，菌株显示的环丙沙星抗性转移比范围为10-4至10-7。在该转移率上未观察到暴露于亚抑制浓度的BKC的显着影响。

Resistance to antibiotics threatens to become a worldwide health problem. An important attributing phenomenon in this context is that pathogens can acquire antibiotic resistance genes through conjugative transfer of plasmids. To prevent bacterial infections in agricultural settings, the use of veterinary hygiene products, such as disinfectants, has gained popularity and questions have been raised about their contribution to such spreading of antibiotic resistance. Therefore, this study investigated the effect of subinhibitory concentrations of benzalkoniumchloride (BKC), a quaternary ammonium compound (QAC), on the conjugative transfer of antibiotic resistance genes. Five Escherichia coli field strains originating from broiler chickens and with known transferable plasmid-mediated ciprofloxacin resistance were exposed to subinhibitory BKC concentrations: 1/3, 1/10 and 1/30 of the minimum bactericidal concentration. Antibiotic resistance transfer was assessed by liquid mating for 4 h at 25°C using E. coli K12 MG1655 as recipient strain. The transfer ratio was calculated as the number of transconjugants divided by the number of recipients. Without exposure to BKC, the strains showed a ciprofloxacin resistance transfer ratio ranging from 10−4 to 10−7. No significant effect of exposure to subinhibitory concentrations of BKC was observed on this transfer ratio.

https://academic.oup.com/ps/article-abstract/98/7/2972/5471359