摘要

猪粪被认为是抗生素抗性基因（ARG）的储存库，可能进入土壤，然后进入食物链，危害人类健康。 这项研究调查了堆肥过程中添加0％，5％和15％（w / w）大孔吸附树脂（MAR）对ARGs和细菌群落的影响。 结果表明，猪粪堆肥处理后，MAR的添加降低了ARGs的丰度（14.14-99.44％）和移动遗传因子（MGEs）（47.83-99.48％）。 在ARG和MGE之间检测到显着的正相关，因此MGE的变化可能导致ARG的变化。 冗余分析表明，MGE对ARG的影响要强于环境因素和细菌群落。 网络分析表明，普通宿主细菌中同时存在ARG和MGE。 总之，结果表明，添加5％MAR可以降低ARG传播的风险。

Swine manure is considered a reservoir for antibiotic resistance genes (ARGs), which may enter the soil and then the food chain to endanger human health. This study investigated the effects of adding 0%, 5%, and 15% (w/w) macroporous adsorption resin (MAR) on ARGs and the bacterial community during composting. The results showed that the addition of MAR reduced the abundances of ARGs (14.14-99.44%) and mobile genetic elements (MGEs) (47.83-99.48%) after swine manure composting. Significant positive correlations were detected between ARGs and MGEs, and thus the variations in MGEs may have led to the changes in ARGs. Redundancy analysis showed that MGEs had stronger effects on ARGs than environmental factors and the bacterial community. Network analysis suggested that ARGs and MGEs co-existed in common host bacteria. In conclusion, the results showed that adding 5% MAR can reduce the risk of ARG transmission.

https://www.ncbi.nlm.nih.gov/pubmed/31634802