摘要

在中国广东沿海地区调查了8种抗生素和21种抗生素耐药基因的浓度。抗生素的总浓度为0.43 ng / L至1040.31 ng / L。在大多数采样点中，四环素的浓度比磺酰胺的浓度高得多。目标抗生素抗性基因的丰度范围为1.82××105至5.9××109拷贝/ mL，tetM占大多数采样点中检测到的抗生素抗性基因的最高百分比。此外，水样中的优势菌群是Proteobacteria，Bacteroidetes和Actinobacteria。还研究了抗生素，抗生素抗性基因和细菌群落之间的关系。结果，sul1的丰度与磺胺嘧啶，磺胺甲恶唑和磺酰胺对甲基氧嘧啶的浓度呈正相关。此外，磺酰胺对甲基氧嘧啶，磺胺嘧啶和对氨基苯磺酰胺与细菌群落显着相关。这些发现表明广东沿海地区的抗生素残留会影响抗生素抗性基因的分布并改变微生物群落。

The concentration of 8 antibiotics and 21 antibiotic resistance genes were investigated in the coastal areas of Guangdong, China. Total concentrations of antibiotics ranged from 0.43 ng/L to 1040.31 ng/L. The concentrations of tetracyclines were much higher than that of sulfonamides in most sampling sites. The abundance of target antibiotic resistance genes ranged from 1.82 × 105 to 5.9 × 109 copies/mL and tetM accounted for the highest percentages of detected antibiotic resistance genes in most sampling sites. Furthermore, the dominant phyla in water samples were Proteobacteria, Bacteroidetes and Actinobacteria. The relationship between antibiotics, antibiotic resistance genes, and bacterial communities was also investigated. As a result, the abundance of sul1 was positively correlated with the concentration of sulfadiazine, sulfamethoxazole and sulfonamide p-methyl oxypyrimidine. Besides, sulfonamide p-methyl oxypyrimidine, sulfadiazine and p-aminobenzenesulfonamide were significantly correlated with the bacterial communities. These findings suggested that the residues of antibiotics in coastal areas of Guangdong affect the distribution of antibiotic resistance genes and alter the microbial communities.

https://www.ncbi.nlm.nih.gov/pubmed/31629908