摘要

     在城市生活垃圾堆肥过程中，抗生素抗性基因（ARGs）可能是阻碍垃圾堆肥应用的重要因素之一。然而，对工业规模堆肥过程中ARGs的富集和传播的认识尚不清楚。因此，本研究旨在探讨工业规模生活垃圾堆肥厂不同阶段的ARG分布。本文研究了7种靶向ARGs和4种靶向可移动基因元件（MGEs）及细菌群落。在两个高温好氧阶段，ARGs和MGEs的丰度都有所增加，但堆肥后大部分ARGs和MGEs的丰度降低。网络分析表明，ARGs的潜在宿主菌主要是厚壁菌门和放线菌。减少潜在的宿主菌对去除ARGs具有重要意义。MGEs是阻碍ARG去除的重要因素。水溶性S和pH是影响微生物群落变化和ARGs丰度的两个主要理化因素。

      During the municipal solid waste (MSW) composting, antibiotic resistance genes (ARGs) could be one of the concerns to hinder the application of MSW composting. However, the understanding of enrichment and dissemination of ARGs during the industrial-scale composting is still not clear. Hence, this study aimed to investigate the ARG distributions at different stages in an industrial-scale MSW composting plant. Seven target ARGs and four target mobile genetic elements (MGEs) and bacterial communities were investigated. The abundances of ARGs and MGEs increased during two aerobic thermophilic stages, but they decreased in most ARGs and MGEs after composting. Network analysis showed that potential host bacteria of ARGs were mainly Firmicutes and Actinobacteria. The reduction of potential host bacteria was important to remove ARGs. MGEs were an important factor hindering ARG removal. Water-extractable S and pH were two main physicochemical factors in the changes of microbial community and the abundance of ARGs.

       https://www.sciencedirect.com/science/article/abs/pii/S0960852419319522