摘要

最近抗生素耐药性的广泛增加已经成为对人类和环境生态系统健康的真正威胁。在储油库中，一个可能受人类活动如回注水影响的极端环境，抗药性基因（ARGs）的分布和丰度，至今仍知之甚少。本文对我国某注水油田不同部位的ARGs的分布进行了研究，发现该系统各部位均存在ARGs。回注水系统的表层更易受ARG污染，最终ARG浓度达到2.2×108基因拷贝/L，其中磺胺类最为丰富。然而，回注水系统地下部分的样品中ARG浓度急剧下降。不同采样点的细菌群落组成也不同。采油井样品中富含原油，细菌较多，但群落丰富度较低。研究还表明，地上污水回收过程可能会对ARGs的扩散造成影响，而地上污水的回收过程旨在减少对环境的直接排放。

The recent widespread increase in antibiotic resistance has become a real threat to both human and environmental ecosystem health. In oil reservoirs, an extreme environment potentially influenced by human activity such as water flooding, the distribution and abundance of antibiotic resistance genes (ARGs) remains poorly understood. Herein, we investigated the distribution of ARGs at different positions in a water-flooding oilfield in China, and found that ARGs were observed in all parts of the investigated system. The surface regions of the water re-injection system were more vulnerable to ARG pollution, and the final ARG concentration was up to 2.2 × 10⁸ gene copies/L, and sulfonamide were the most abundant. However, ARG concentration decreased sharply in the samples from underground part of the re-injection system. The bacterial community composition was also varied with sampling position. The sample from production well, which was enriched in crude oil, contained more bacteria but the community richness was simpler. This study also indicated the wastewater-recycling process above ground, which proposed to reduce the discharge into environment directly, may pose a risk for ARGs spread.

https://www.sciencedirect.com/science/article/pii/S0147651319314241