摘要

本研究用qPCR法测定了6种ARGs（tetX、sul1、qnrS、blaTEM、ermB和intl1），同时测定了无机氮（氨氮、亚硝酸盐、硝酸盐）、总磷（TP）和化学需氧量（COD）。污水处理设施对6个目标基因有消除作用，绝对丰度从进水的104-107拷贝/mL下降到出水的103-105拷贝/mL，去除率为74.27-96.51%，对tetX的去除率最高。去除ARGs的主要处理单元为曝气池和二沉池。出水和下游水体中ARGs的绝对丰度分别是上游水体的3.24-18.83和1.86-8.55倍。不同目标的ARGs绝对丰度呈正相关（R=0.6762±0.1777），说明ARGs清除机制相似，ARGs与氨氮呈正相关（R=0.5025±0.2711）。未经处理的废水含有大量的ARGs，部分被污水处理厂去除。但出水中ARGs绝对丰度较高，导致受纳河流水相ARGs增加。因此，污水是受纳河流的重要污染点源。

Six ARGs (tetX, sul1, qnrS, blaTEM, ermB and intl1) were quantified by qPCR, along with concentrations of inorganic nitrogen (ammonia, nitrite, nitrate), total phosphorous (TP) and chemical oxygen demand (COD). The sewage treatment facilities had elimination effects on the six target genes; absolute abundance decreased from 10⁴–10⁷ copies/mL in the influent to 10³–10⁵ copies/mL in effluent, and the removal efficiencies were 74.27–96.51%; the highest removal performance was for tetX. The main treatment units for eliminating ARGs were the aeration tank and the secondary sedimentation tank. Absolute abundances of ARGs in the effluent and downstream water were 3.24–18.83 and 1.86–8.55 times higher than that in the upstream river, respectively. The absolute abundances of different target ARGs were positively correlated (R = 0.6762 ± 0.1777), indicating similar elimination mechanisms, and a positive correlation between ARGs and ammonia nitrogen (R = 0.5025 ± 0.2711). Raw wastewater contained numerous ARGs, which were partially removed by the WWTP. However, there remained a high absolute abundance of ARGs in effluent, causing an increase in water-phase ARGs in the receiving river. Hence, effluent was an important pollution point source for the receiving river.

https://iopscience.iop.org/article/10.1088/1755-1315/435/1/012012/meta