摘要

      分析了4个污水处理厂（WWTPs）、2个污水接收区（era）和杭州湾（HZB）中14个ARGs、intI1和16srdna的分布。结果表明，各综合污水处理厂（IWWTP）的ARGs丰度均高于制药污水处理厂（PWWTPs），且IWWTPs对ARGs的去除效果优于PWWTPs。污水处理厂排放的废水对ERAs和HZB水环境中的ARGs污染有很大的贡献，且ARGs总丰度呈距离衰减规律。在海岸沉积物中，更多的泥质沉积在偏远地区。相关分析表明，在污水处理厂中ARGs的发生与16srdna和intI1的关系更为密切。三个大环内酯类抗药性基因（ermB、mphA和vatB）与所有样本组的16srdna和intI1有很强的相关性。我们的研究清楚地揭示了陆地污水处理厂的排放与沿海环境中出现的ARGs污染之间的联系。

      The distribution of 14 ARGs, intI1, and 16S rDNA were analysed in 4 wastewater treatment plants (WWTPs), 2 effluent receiving areas (ERAs), and Hangzhou Bay (HZB). The results showed that each integrated WWTP (IWWTP) received higher abundance of ARGs than pharmaceutical WWTPs (PWWTPs), and IWWTPs removed ARGs more efficiently than PWWTPs. The WWTP effluents greatly contributed to the ARGs pollution in the water environments of the ERAs and HZB, and the total abundance of the ARGs displayed a distance decay pattern. In coastal sediments, more ARGs were accumulated in remote sites. The correlation analysis showed that the occurrence of ARGs was more related to 16S rDNA and intI1 in the WWTPs. Three macrolides resistance genes (ermB, mphA, and vatB) had strong correlations with 16S rDNA and intI1 in all the sample groups. Our study clearly reveals the link between land WWTPs discharge and emerging pollution of ARGs in coastal environments.

      https://linkinghub.elsevier.com/retrieve/pii/S0025-326X(19)31012-4