摘要

      我们从两个不同的地理区域分析了埃塞俄比亚本地鸡盲肠微生物群的全基因组：阿法尔（AF）区（Dulecha，海拔730m）和阿姆哈拉（AM）区（Menz Gera Midir，海拔3300 m）。通过亚基因组分析发现，微生物种群以类杆菌和硬壁菌为主。我们鉴定了两组共2210个基因。LEfSe结果表明，AF中的协杆菌、地杆菌、克罗诺巴菌、异普氏菌和失调单胞菌的分布较AM丰富。利用KEGG、eggNOG和CAZy数据库进行的分析表明，代谢、遗传信息处理、环境信息处理和细胞过程的途径显著丰富。功能丰度与本地鸡的营养吸收和微生物定位有关。我们还研究了抗生素耐药基因，发现像LSM、头孢菌素、四环素等抗生素在AF中的含量明显高于AM。

       We analyzed the whole genomes of cecum microbiomes of Ethiopian indigenous chickens from two distinct geographical zones: Afar (AF) district (Dulecha, 730 m above sea level) and Amhara (AM) district (Menz Gera Midir, 3300 m). Through metagenomic analysis we found that microbial populations were mainly dominated by Bacteroidetes and Firmicutes. We identified 2210 common genes in the two groups. LEfSe showed that the distribution of Coprobacter, Geobacter, Cronobacter, Alloprevotella, and Dysgonomonas were more abundant in AF than AM. Analyses using KEGG, eggNOG, and CAZy databases indicated that the pathways of metabolism, genetic information processing, environmental information processing, and cellular process were significantly enriched. Functional abundance was found to be associated with the nutrient absorption and microbial localization of indigenous chickens. We also investigated antibiotic resistant genes and found antibiotics like LSM, cephalosporin, and tetracycline were significantly more abundant in AF than AM.

        https://www.sciencedirect.com/science/article/pii/S0888754319305774