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营养能调控对污泥堆肥过程中抗生素抗性基因命运的影响

发布者:抗性基因网 时间:2020-03-20 浏览量:641

      摘要

      污泥堆肥作为一种土壤营养改良剂,因其最终产品的应用而得到越来越多的应用。尽管C/N比值在堆肥质量和堆肥过程中具有重要意义,但营养能(碳和氮)对污泥堆肥过程中抗生素抗性基因(ARGs)命运的影响却鲜有报道。研究了污泥堆肥过程中ARGs、微生物群落及功能特性的动态变化。研究了三种碳氮比(20:1、25:1和30:1)对秸秆发酵污泥堆肥的影响,并以污泥为对照(C/N=9.5:1)。本工作的一个新发现是,最高的初始C/N比(30:1)可以延长高温期,这有助于减少一些靶ARGs。部分ARGs(sul1、sul2、aadA1)与多种代谢途径呈负相关,难以清除。


      Sludge composting is increasingly adopted due to its end product for application as a soil nourishment amendment. Although the ratio of C/N is significant in the quality and process of composting, little information has been obtained from the effects of nutritional energy (carbon and nitrogen) on the fate of antibiotic resistance genes (ARGs) during sludge composting. Dynamic variations of ARGs, microbial community as well as functional characteristics during composting of sludge were investigated in this study. Three levels of carbon to nitrogen (20:1, 25:1 and 30:1) were developed for the composting of sludge with fermented straw plus a control which was just sewage sludge (C/N = 9.5:1). A novel finding of this work is that the highest initial C/N ratio (30:1) could prolong the thermophilic period, which was helpful to reduce some target ARGs. Some ARGs (sul1, sul2, and aadA1) had negative correlation with multiple metabolic pathways, which were difficult to remove.

      https://www.sciencedirect.com/science/article/abs/pii/S0960852419317432