摘要

      新兴的抗药性细菌（ARB）和抗药性基因（ARGs）越来越被认为与微生物污染物一样重要。研究了紫外线活化过硫酸盐（UV/PS）深度氧化法去除二级废水中的ARB和ARGs。结果表明，UV/PS对大环内酯类耐药菌（MRB）、磺胺类耐药菌（SRB）、四环素类耐药菌（TRB）和喹诺酮类耐药菌（QRB）的灭活率分别为96.6%、94.7%、98.0%和99.9%。UV/PS对ARGs也有明显的去除效果。在UV/PS条件下，总ARGs的减少量达到3.84个数量级，比UV条件下减少了0.56log。特别是UV/PS法去除了可能有利于ARGs在不同微生物间水平基因转移的移动遗传元素（MGE），达到76.09%，高通量测序结果表明UV/PS法改变了微生物群落。蛋白质细菌和放线菌对人体健康的危害分别比紫外线低4.25%和1.6%。共现分析表明，ARGs在细菌类群中的贡献是不同的。在UV/PS体系中，羟基自由基和硫酸根有助于细菌和ARGs的去除。本研究为UV/PS去除废水中的ARGs和ARB提供了一种新的方法。

      The emerging antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are increasingly appreciated to be as important as microbial contaminants. This paper focused on UV-activated persulfate (UV/PS), an advanced oxidation process, in removing ARB and ARGs from secondary wastewater effluent. Results showed that the inactivation efficiency of macrolides-resistant bacteria (MRB), sulfonamides-resistant bacteria (SRB), tetracyclines-resistant bacteria (TRB) and quinolones-resistant bacteria (QRB) by UV/PS reached 96.6 %, 94.7 %, 98.0 % and 99.9 % in 10 min, respectively. UV/PS also showed significant removal efficiency on ARGs. The reduction of total ARGs reached 3.84 orders of magnitude in UV/PS which is more than that in UV by 0.56 log. Particularly, the removal of mobile genetic elements (MGE) which might favor the horizontal gene transfer of ARGs among different microbial achieved 76.09 % by UV/PS. High-throughput sequencing revealed that UV/PS changed the microbial community. The proportions of Proteobacteria and Actinobacteria that pose human health risks were 4.25 % and 1.6 % less than UV, respectively. Co-occurrence analyzes indicated that ARGs were differentially contributed by bacterial taxa. In UV/PS system, hydroxyl radical and sulfate radical contributed to the removal of bacteria and ARGs. Our study provided a new method of UV/PS to remove ARGs and ARB for wastewater treatment.

      https://www.sciencedirect.com/science/article/abs/pii/S030438942030056X