摘要

        水中出现的耐万古霉素粪肠球菌（E.faecalis）威胁着人类的健康。本文研究了紫外线消毒对耐万古霉素粪肠球菌的影响，包括对粪肠球菌的作用、光激活和暗修复，以及其失活机理。紫外线消毒能迅速灭活目标抗生素耐药菌（ARB）、粪大肠杆菌，对细胞膜造成损伤，导致三磷酸腺苷（ATP）和超氧化物歧化酶（SOD）活性显著降低（p<0.05）。E、 紫外线消毒后，尤其是在光照条件下，粪便可以重新激活。此外，还研究了紫外线照射对粪肠球菌筛选的抗生素耐药基因（ARG）、万古霉素（vanB）的去除及对万古霉素耐药的影响，结果表明紫外线消毒对粪肠球菌万古霉素耐药无显著影响（p>0.05）。

        The emergence of vancomycin-resistant Enterococcus faecalis (E. faecalis) in water is threatening the health of human beings. The effect of ultraviolet disinfection on vancomycin-resistant E. faecalis, including the effectiveness, photoreactivation and dark repair of E. faecalis, and the deactivation mechanism were investigated in this work. Ultraviolet disinfection could quickly inactivate the target antibiotic resistant bacterium (ARB), E. faecalis, and it caused damage to the cell membrane and induced the decrease of the total adenosine triphosphate (ATP) content and the superoxide dismutase (SOD) activity significantly (p < 0.05). E. faecalis could reactivate after ultraviolet disinfection especially under light conditions. Furthermore, the removal of the selected antibiotic resistance gene (ARG), vanB, by ultraviolet radiation and the effect on the vancomycin resistance of E. faecalis were investigated, which showed that ultraviolet disinfection had no significant effect on the vancomycin resistance of E. faecalis (p > 0.05).

         https://pubs.rsc.org/en/content/articlelanding/2020/EM/C9EM00435A#!divAbstract