摘要

       矿物和微生物是自然环境的组成部分，它们不可避免地相互作用。抗生素耐药基因（ARGs）严重威胁着现代医疗保健。然而，天然矿物对水体中ARG繁殖的影响还不完全清楚。研究了天然闪锌矿（NS）纳米粒子对大肠杆菌DH5α（CTX）（供体）和大肠杆菌DH5α（MCR）（供体）水平转移ARGs的影响及其机制。NS颗粒（0.5-50mg L-1）诱导了NS浓度依赖的共轭转移频率的增加。促进ARG转移的机制包括细胞内活性氧的产生、SOS反应、细菌细胞形态的改变、细菌细胞膜蛋白相关基因和结合ARG转移相关基因mRNA水平的改变。本文的信息提供了新的机制性理解的风险，细菌耐药性造成的NS。

       Minerals and microorganisms are integral parts of natural environments, and they inevitably interact. Antibiotic-resistance genes (ARGs) significantly threaten modern healthcare. However, the effects of natural minerals on ARG propagation in aquatic systems are not fully understood. The present work studied the effects of natural sphalerite (NS) nanoparticles on the horizontal transfer of ARGs from Escherichia coli DH5α (CTX) (donor) to E. coli C600 (Sm) (recipient), and from E. coli DH5α (MCR) (donor) to E. coli C600 (Sm), and their underlying mechanisms. NS particles (0.5-50 mg L^-1) induced an NS-concentration-dependent increase in conjugative transfer frequency. The underlying mechanisms associated with the facilitated ARG transfer included the production of intracellular reactive oxygen species, the SOS response, changes in bacterial cell morphology, and alteration of mRNA levels of bacterial cell membrane protein-related genes and genes associated with conjugative ARG transfer. The information herein offers new mechanistic understanding of risks of bacterial resistance resulting from NS.

       https://www.sciencedirect.com/science/article/pii/S0160412019345039?via%3Dihub