摘要

       海洋是人类活动产生的污染物的最终沉积地。因此，海洋的长距离运输可以促进包括ARGs在内的陆地污染物的扩散。然而，据我们所知，目前对陆生arg的含量变化及海岸带变化原因的研究还很少。本研究建立了不同比例的海水与淡水混合的微观模型，模拟了沿海地区不同区域的环境条件。对4个ARGs进行了定量，并进行了16S焦磷酸测序。结果表明，陆生ARG对海岸带相应ARG的含量有影响，各ARG含量变化规律明显。在大多数情况下，盐度对ARG含量的影响是有限的。除小蠊外，大部分淡水优势菌与所选arg呈显著正相关（p<0.05）。在海水比例较高的微观环境中，淡水中的优势菌数量急剧减少。淡水可能对海水中的细菌组成产生强烈的影响，淡水中的物质可能促进一些细菌（包括潜在的ARGs宿主）在沿海地区的生长。

        The ocean is the final place where pollutants generated by human activities are deposited. As a result, the long-range transport of the ocean can facilitate the diffusion of terrestrial contaminants, including ARGs. However, to our knowledge, little research has been devoted to discussing the content change of terrestrial ARGs and the reason for the change in coastal area. This study established various microcosms, in which seawater and freshwater were mixed at different ratio to simulate the environmental conditions of different regions in coastal areas. Four ARGs were quantified, and 16S pyrosequencing was conducted. The results showed that the terrestrial ARGs influenced the concentration of the corresponding ARGs in coastal areas, and the content change pattern of each ARG was distinct. The influence of salinity on the ARG content was limited in most cases. Moreover, most dominant bacteria from freshwater had significant positive correlation (p < 0.05) with selected ARGs, except for bla_TEM. The dominant bacteria in freshwater diminished dramatically in microcosms with a high proportion of seawater. Freshwater may have a strong impact on the bacteria composition of seawater, and the materials from freshwater may prompt the growth of some bacteria (include potential hosts of ARGs) in coastal area.

        https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(18)30331-X