摘要

        随着我国海水养殖的快速发展，大量的抗生素被排放到水环境中。关于中国海水养殖环境中的抗生素和相应的抗生素耐药基因（ARGs）的资料很少。收集了我国沿海11个典型海水养殖区的底泥，并以南方梅鸡角的底泥为非海水养殖对照。结果表明，抗生素及其相应的精氨酸广泛分布于养殖底泥中，且在梅鸡角样品中含量较低。抗磺胺类药物基因普遍存在，以蓬莱地区的sul1和sul2为最高。此外，不同地点间精氨酸含量也有显著差异。由于检测ARGs的引物有限，本研究还采用illumina高通量测序法对ARGs进行了分析，结果表明，所有样品中bacA的丰度最高，其次是mexF和mexB。这是首次对我国沿海典型海水养殖区的抗生素耐药性进行综合调查。本研究为了解海水养殖对我国细菌和精氨酸组成的影响提供了依据。

        With the rapid development of mariculture in China, large amounts of antibiotics are being discharged into the aquatic environment. Little information is available regarding antibiotics and corresponding antibiotic resistance genes (ARGs) associated with maricultural environments in China. Sediments from eleven typical mariculture areas along the whole coastline of China were collected, and the sediment in Meijijiao in southern China was used as a non-mariculture control. The results revealed that antibiotics and their corresponding ARGs were widely distributed in most maricultural sediments, and present at low concentrations in samples from Meijijiao. The sulfonamide-resistance genes were prevalent, and the sul1 and sul2 in Penglai were the highest detected by using quantitative PCR. Moreover, remarkable differences in ARGs among different sites were observed. Due to the limited availability of primers to detect ARGs, illumina high-throughput sequencing was also used for profiling ARGs, and the results showed that the abundance of bacA in all samples was the highest compared to other ARGs, followed by mexF and mexB. This is the first study to comprehensively investigate the antibiotic resistance profile in typical mariculture areas along the whole coast of China. This study provides insights into the impacts of mariculture on the profiles of bacterial and ARG compositions in China.

        https://www.sciencedirect.com/science/article/pii/S0048969718305126?via%3Dihub