摘要

       生物炭对厌氧消化过程中的氮素保存有积极作用，但其对抗生素抗性基因（ARGs）的影响尚不清楚。因此，研究了生物炭（0、5、20和50 g/L）对牛粪废水厌氧消化过程中ARGs环境风险的影响。结果表明，5 g/L生物炭降低了5/13 ARGs的相对丰度（RAs），20 g/L生物炭显著降低了消化产物中ARGs的总RAs，其中ISCR1的RA比对照低0.89log。生物炭主要通过影响厚壁菌和蛋白菌RAs来影响ARGs的分布，20 g/L生物炭对ARG的影响大于5 g/L生物炭，而移动遗传因子也影响ARG的分布，尤其是intI2和ISCR1。在牛场废水厌氧消化系统中添加20 g/L生物炭，可以降低ARGs的环境风险。

       Biochar has positive effects on nitrogen conservation during anaerobic digestion, but its impacts on antibiotic resistance genes (ARGs) are unclear. Therefore, the effect of biochar (0, 5, 20, and 50 g/L) on the environmental risk of ARGs during cattle manure wastewater anaerobic digestion were investigated. The results showed that 5 g/L biochar reduced the relative abundances (RAs) of 5/13 ARGs while 20 g/L biochar significantly reduced the total RAs of ARGs in the digestion products, where the RA of ISCR1 was 0.89 log lower than the control. Biochar mainly affected the distribution of ARGs by influencing the RAs of Firmicutes and Proteobacteria, and the influence of 20 g/L biochar was greater than that of 5 g/L. Mobile genetic elements also influenced the ARG profiles, especially intI2 and ISCR1. The addition of 20 g/L biochar to cattle farm wastewater anaerobic digestion systems could reduce the environmental risk of ARGs.

       https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(18)30236-0