摘要

本研究采用宏基因组学方法，探讨蚯蚓对脱水污泥蚯蚓堆肥过程中ARGs和HPB的影响。结果表明，污泥蚯蚓粪中含有139种ARGs，隶属于30个类群。与对照相比，污泥蚯蚓粪中ARGs的总丰度降低了41.5%。此外，蚯蚓堆肥也降低了质粒和整合子的类型和序列。蛋白菌和放线菌是污泥蚯蚓中ARGs的主要宿主。此外，蚯蚓降低了总HPB的丰度，改变了它们的多样性，从而导致污泥蚯蚓粪中肠杆菌科的丰度较高。然而，污泥蚯蚓堆肥仍然富含精氨酸和羟丙基丁酸，表明农业用途的环境风险仍然存在。蚯蚓活动引起的微生物群落变化和可移动遗传元素的减少是减轻蚯蚓堆肥过程中ARG污染的主要原因。

      This study used a metagenomic approach to investigate the effects of earthworms on ARGs and HPB during the vermicomposting of dewatered sludge. Results showed that 139 types of ARGs were found in sludge vermicompost, affiliated to 30 classes. Compared with the control, the total abundance of ARGs in sludge vermicompost decreased by 41.5%. Moreover, the types and sequences of plasmids and integrons were also decreased by vermicomposting. Proteobacteria and Actinobacteria were the most dominant hosts of ARGs in sludge vermicompost. In addition, earthworms reduced the total HPB abundance and modified their diversity, thus leading to higher abundance of Enterobacteriaceae in sludge vermicompost. However, the sludge vermicompost was still ARG and HPB enriched, indicating a remaining environmental risk for agricultural purpose. The observed change of microbial community and the reduction of mobile genetic elements caused by earthworm activity are the main reasons for the alleviation of ARG pollution during vermicomposting.

       https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(19)31681-5