摘要

      由于其对人类健康的严重威胁，环境中的抗微生物性越来越受到人们的重视。城市污水处理厂出水被认为是抗生素耐药基因（ARGs）传播的重要来源之一。然而，传统的消毒技术不能有效地去除废水中的ARGs。本文首次将原位合成水合氧化锰（HMO）与高锰酸钾（高锰酸盐）联用，用于污水处理厂出水中的精氨酸（ARG）去除。结果表明，5种ARGs（sulI、sulII、tetQ、tetO和tetW）以及intI1和16srrna基因的去除率为2.46-4.23logs，明显高于单独使用这些试剂的去除率。这表明高锰酸盐与HMO对ARGs的去除有协同作用。此外，对HMO混凝和高锰酸盐氧化对ARG去除的贡献进行了半定量研究，结果表明，氧化破坏微生物细胞和去除混凝释放的胞外ARG是该系统的两个主要过程。研究结果为废水处理厂废水中ARGs的高效去除提供了一种新的方法，以控制ARGs的扩散。

      An increasing amount of attention has been given to antimicrobial resistance in the environment because of its substantial threat to human health. The effluent from municipal wastewater treatment plants has been regarded as one of the important sources for the spread of antibiotic resistance genes (ARGs). However, conventional disinfection techniques fail to effectively remove ARGs from effluents. In this work, in situ synthesized hydrated manganese oxide (HMO) coupled with permanganate was applied for the first time in ARG removal from the effluent of wastewater treatment plants. The results show that five ARGs (sulI, sulII, tetQ, tetO, and tetW) as well as the intI1 and 16S rRNA genes had removal efficiencies of 2.46-4.23 logs, which were significantly higher than those obtained by using these reagents individually. This implied that there was a synergistic effect between permanganate and HMO toward the removal of ARGs. Moreover, the contributions of HMO coagulation and permanganate oxidation to ARG removal were semiquantitatively studied, which demonstrated that destruction of the microbial cells by oxidation and removal of the extracellular ARGs released by coagulation were the two main processes in this system. The results of this study provide an alternative method for ARG removal from the effluent of wastewater treatment plants with high efficiencies to control the spreading of ARGs.

       https://pubs.acs.org/doi/abs/10.1021/acs.est.9b05250