摘要

      由质粒介导的AMR基因（ARGs）编码的AMR是一种日益严重的全球性公共卫生威胁。野生动物作为ARGs的哨兵、水库和潜在的载体发挥着基础性的作用。在加拉帕戈斯，我们首次确定并量化了在自由生活的巨型龟（Chelonoidis porteri）中存在的ARG。我们对分布在圣克鲁斯岛的30只乌龟泄殖腔中的粪便进行了ARG定量PCR分析。验证样本（n=28）由多达21个不同的ARGs组成的小组进行分析，所有28个乌龟样本对一个或多个编码抗性的基因均呈阳性。21只被测试的乌龟中有13只出现在至少一个样本中，10只乌龟（35.7%）有多重抗性模式。我们建议进行更多的研究，以便更全面地了解加拉帕戈斯群岛各分类群和地理位置的抗性模式，以及ARGs对野生动物、家畜和人类健康的影响。在这项研究中，我们发现100%的取样巨龟的粪便中含有ARGs，这表明这些基因在群岛内有大规模的分布。

       Antimicrobial resistance (AMR), encoded by plasmid-mediated AMR genes (ARGs), is an increasing global public health threat. Wildlife play a fundamental role as sentinels, reservoirs and potential vectors of ARGs. For the first time in Galapagos, we have identified and quantified the presence of ARGs in free-living giant tortoises (Chelonoidis porteri). We performed ARG analyses by quantitative PCR of faeces collected from the cloaca of 30 tortoises widely distributed across Santa Cruz Island. Validated samples (n = 28) were analysed by a panel of up to 21 different ARGs and all 28 tortoise samples were positive to one or more genes encoding resistance. Thirteen of 21 tested ARGs were present in at least one sample, and 10 tortoises (35.7%) had a multi-resistant pattern. We recommend additional research so we may more fully understand resistance patterns across taxa and geographical locations throughout the Galapagos archipelago, and the implications of ARGs for the health of wildlife, domestic animals, and humans. In this study, we found 100% of sampled giant tortoises had ARGs present in their faeces, suggesting a large-scale distribution of these genes within the archipelago.

       https://onlinelibrary.wiley.com/doi/abs/10.1111/zph.12639